磷脂酰肌醇-3激酶活性在妊娠期糖尿病患者脂肪组织中的检测  

Analysis of phosphatidylinositol 3-kinase activity in the adipose tissue of gestational diabetes mellitus patients

作　　者：初永丽[1] 刘文娟[2] 崔青[1] 冯桂姣[1] 王彦[1] 姜学强[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院妇产科,264000 [2]烟台市莱阳中心医院科教科

出　　处：《中国妇产科临床杂志》2010年第3期210-212,共3页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：山东省优秀青年科学家基金项目(NO:2006BS03053)资助

摘　　要：目的检测妊娠期糖尿病(gestational diabetes mellitus,GDM)患者脂肪组织磷脂酰肌醇-3激酶(phosphatidylinositol 3-kinase,PI-3K)活性的改变,探讨GDM产生胰岛素抵抗(insulin resistance,IR)的分子机制。方法采用放射免疫法测定35例正常妊娠妇女及35例GDM患者血清空腹胰岛素(fasting insu-lin,FIN)的浓度,葡萄糖氧化酶法测定血浆空腹血糖(fasting plasma glucose,FPG);免疫沉淀、薄层层析及γ液闪计数方法检测PI-3激酶的活性,并进行分析比较。结果 GDM组患者FPG[(7.85±3.51)mmol/L]、FIN[(25.17±3.76)mU/L]及HOMA-IR(1.75±0.12)均显著高于对照组,差异有统计学意义(P均<0.01);与对照组比较,GDM组PI-3K活性(85.75%)下降(P<0.01);GDM组PI-3K与孕晚期体重、孕晚期体重指数及HOMA-IR呈负相关(r=-0.59、r=-0.63、r=-0.74,P均<0.01)。结论 GDM患者PI-3K参与了胰岛素信号通路中信号分子的生物学作用,导致IR的形成。Objective To detect the phosphatidylinositol 3-kinase activity in adipose tissue of diabetes mellitus（GDM） patients,thus to explore the molecular mechanisms of insulin resistance of GDM.Methods Samples from patients with GDM（GDM group,n=35）,and healthy pregnant women（controls group,n=35） were collected.Fasting insulin（FIN） was measured by radioimmunoassay.Fasting plasma glucose（FPG） was determined by oxidase assay.PI-3 kinase activity was detected by immunoprecipitation,thin-layer chromatography and gamma scintillation counting.The results were analyzed statistically.Results The levels of serum FPG[（7.85±3.51） mmol/L],FIN [（25.17±3.76） mU/L] and HOMA-IR（1.75±0.12） in GDM group were significantly higher than those in control group（P〈0.01）;PI-3 kinase activity（85.75%） was significantly decreased in GDM group compared with control group（P〈0.01）and negatively correlated with HOMA-IR（r=-0.74,P〈0.01）.Conclusions PI-3 kinase may affect the insulin signaling pathway,and takes part in the insulin resistance of gestational diabetes mellitus.

关 键 词：妊娠期糖尿病 胰岛素抵抗 磷脂酰肌醇-3激酶 

分 类 号：R714.2[医药卫生—妇产科学] R587.1[医药卫生—临床医学]