迟缓爱德华氏菌的溶血特性  

Hemolytic Property of Edwardsiella Tarda

作　　者：葛艳[1] 陈怀青[1] 陆承平[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《中国预防兽医学报》1999年第1期4-6,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省九五重点攻关项目

摘　　要：分析了不同来源的２８株Ｅｔ的溶血特性。分别以平板法（ＰｌａｔｅＡｓｓａｙ，ＰＡ）、接触法（ＣｏｎｔａｃｔＨｅｍｏｌｙｓｉｓ，ＣＨ）及上清法（ＳｕｐｅｒｎａｔａｎｔＡｓａｙ，ＳＡ）检测Ｅｔ的溶血性，阳性率依次为７５％、７５％、５７１４％。ＰＡ和ＣＨ的符合率为１００％。凡ＳＡ检测为阳性的，ＰＡ、ＣＨ检测皆为阳性。培养基中加入螯合剂ＥＤＤＡ造成环境缺铁，结果，约３２１４％Ｅｔ的胞外溶血素显著增加。经胰酶、热处理后，ＡＴＣＣ１５９４７株溶血活性丧失，提示Ｅｔ溶血素是一种不耐热蛋白样物质。The hemolytic activities of 28 Edwardsiella tarda(Et)strains from different sources were investigated.21 strains produced a hemolusin when assayed by either plate assay(PA)or contact hemolysis(CH),while just 16 strains were hemolytic by supernatant assay(SA).And the agreemental rate between PA and CH was 100%.SA could be replated by PA or SA.Hemolytic activities of 9 strains growing in L broth supplemented with EDDA was apparently higher when compared to in L broth alone.Preliminary characterization of the hemolysin of strain ATCC15947 indicated that it was a heat labile proteinaceous subtance.

关 键 词：迟缓爱德华氏菌 溶血素 败血症 鱼类 

分 类 号：S941.42[农业科学—水产养殖]