冠心Ⅱ号不同组分配伍对体外血栓形成、血小板聚集和镇痛作用的影响  

Effects of different Compositions of Guanxin Ⅱ (GXⅡ) Prescription on thrombosis in vitro, platelet aggregation and analgesic effect

作　　者：张金艳[1] 李贻奎[1] 赵乐[1] 李连达[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中药药理与临床》2010年第1期1-3,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究973计划项目(2005CB523405)

摘　　要：目的：分别考察冠心Ⅱ号全方、全方中的活血药和全方中的理气药对大鼠体外血栓形成、血小板聚集以及对小鼠镇痛作用的影响,探讨冠心II号方剂配伍规律。方法：大鼠连续灌胃给药7天后,麻醉,腹主动脉取血,用体外血栓形成仪制作血栓并测量其长度、湿重和干重;用血小板聚集仪测定小板聚集率。小鼠连续灌胃给药4~5天后,分别用腹腔注射醋酸法和热板致痛法制造小鼠疼痛模型,记录小鼠醋酸致痛的扭体潜伏期和扭体次数,及热板致痛的痛阈值。结果：与蒸馏水对照组相比,全方组和活血药组的体外血栓长度,血栓干、湿重,及血小板聚集率均显著降低（P〈0.01或P〈0.05）,二者作用强度无显著性差异;而理气药组的上述各指标与蒸馏水对照组比较略有降低趋势,但无统计学差异。在镇痛试验中,与蒸馏水对照组相比,冠心Ⅱ号全方组和理气药组小鼠的扭体次数显著减少、热板致痛的痛阈值显著增高,且两组作用强度无统计学差异;而活血药组的上述各指标与蒸馏水对照组相比无统计学差异。结论：冠心Ⅱ号全方中发挥抗血栓形成和血小板聚集作用的主要是方中的活血药,理气药在该作用上不起主导作用;而冠心Ⅱ号全方中发挥镇痛作用的则主要是理气药,活血药起到一定的辅助作用。Objective ：To compare effects of the whole prescription of GXII,the hemorheologic agents and the agents regulating QI of the whole prescription on thrombosis in vitro,platelet aggregation and analgesic effect.Method ：Intragastric administration were given to rats once a day for seven days.Blood were obtained through abdominal aorta,thrombus were made via thrombosis equipment in vitro,and then measured its length,wet and dry weight.And also,the platelet aggregation rates were measured.In the pain relieving study,the prophylactic treatments were administered to mice once a day for 4 to 5 days,the response latency and the number of abdominal contractions after the stimulus of intraperitoneal injection of acetic acid,and the pain threshold on hot plate were recorded.Results：Compared to the control,both the whole prescription of GXII and the hemorheologic agents significantly reduced the length,the wet and dry weight of the thrombus,and the platelet aggregation rates.And they have similar efficacy.However,the agents regulating QI of the whole prescription did not show any effect on above parameters.In the pain relieving study,the whole prescription of GXII and the agents regulating QI of the whole prescription significantly reduced the abdominal contractions induced by the intraperitoneal injection of acetic acid,and significantly prolonged the response latency induced by hot plate.And they have similar efficacy.However,the hemorheologic agents did not show significant difference compared with control.Conclusion ：In the whole prescription of GXII,the hemorheologic agents mainly contributed to inhibition of thrombosis and platelet aggregation,and the agents regulating QI of the whole prescription mainly contributed to its analgesic effect.

关 键 词：冠心Ⅱ号 中药配伍 体外血栓 血小板聚集 镇痛 

分 类 号：R285.5[医药卫生—中药学]