重组人粒细胞集落刺激因子对8Gy γ射线照射大鼠凝血系统的影响  

Effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on the coagulation system of 8 Gy gamma-irradiated Wistar rats

作　　者：韩琴芳[1,2] 柳晓兰[2] 赵振虎[2] 丛悦[2] 余祖胤[2] 从玉文[2] 周新文[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215123 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2010年第2期115-118,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的探讨造血细胞因子重组人粒细胞集落刺激因子(rhG-CSF)对8Gyγ射线照射大鼠凝血系统的影响。方法分析照射对照组和rhG-CSF治疗组照射前、照射后3、7、11、15、21和30d的血常规、血小板聚集、凝血四项以及血栓弹力图(TEG)的变化。结果rhG-CSF治疗组动物活存率增加,外周血白细胞、红细胞和血小板数目较照射对照组明显升高(P<0.05);发现rhG-CSF治疗可逆转辐射后活化部分凝血活酶时间(APTT)缩短(P<0.05),加快TEG血栓形成速度,增加血栓形成最大幅度(P<0.05)。结论rhG-CSF能明显促进8Gyγ射线照射大鼠造血功能恢复,并改善辐射后凝血功能异常。Objective To explore the effect of recombinant human granulocyte colony-stimulating factor（rhG-CSF）on the coagulation parameters of Wistar rats irradiated with a single dose of 8 Gy γ-ray from cobalt-60.Methods Clotting tests and thrombelastogram（TEG）were used to examine the effect of rhG-CSF on the coagulation parameters.A blood cell counter was applied to count the blood cells.A platelet aggregometer was adopted to observe the platelet aggregation.Results Compared with irradiated control group,white blood cells and red blood cells of peripheral blood as well as the platelet number were increased（P0.05）,platelet aggregation was strengthened,the velocity of thrombosis was improved,and the maximum amplitude（MA）of the TEG was raised in rhG-CSF treated group（P0.05）.Conclusion rhG-CSF can significantly promote the recovery of hemopoiesis and reverse the abnormal changes of the coagulation system in rats irradiated by the cobalt-60 with a single dose of 8 Gy.

关 键 词：重组人粒细胞集落刺激因子 Γ射线 血栓弹力图 凝血四项 造血细胞生长因子 

分 类 号：R817[医药卫生—影像医学与核医学]