雷米普利对糖尿病心肌梗死大鼠葡萄糖转运体4的影响  

Effects of Ramipril on the ventricular remodeling and GLUT4 in diabetic rats complicated with myocardial infarction

作　　者：张瑞英[1] 王玉梅[1] 王斐[1] 沈景霞[1] 于萍[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心内科,哈尔滨150001

出　　处：《中华急诊医学杂志》2010年第5期488-492,共5页Chinese Journal of Emergency Medicine

基　　金：基金项目：黑龙江省攻关课题资助项目（GC05C40304）;哈尔滨市学科后备带头人基金项目资助（2005AFXXJ047）

摘　　要：目的观察2型糖尿病合并心肌梗死大鼠心室重构及葡萄糖转运体（GLIT4）表达及雷米普利对其影响。方法46只雄性Wistar大鼠由哈尔滨医科大学第一临床医学院实验动物中心提供。高脂喂养加低剂量链脲佐菌素（STZ，30mg／kg）腹腔注射，血糖值≥16．7mmol／L为2型糖尿病模型，结扎冠状动脉左前降支制备心肌梗死模型；糖尿病合并心肌梗死大鼠随机（随机数字法）分为安慰剂组（n=9），雷米普利组（n=10），糖尿病假手术组（n=10）；非糖尿病大鼠随机分为心肌梗死组（n=9）及非糖尿病假手术组（n=8）。术后6周查心脏超声、测定心室重量指数、心肌梗死面积及左室非梗死区心肌胶原容积分数。取大鼠非梗死区心肌，采用实时定量RT-PCR技术测定GLUT4mRNA，Western blot法测定GLUT4蛋白含量。用SPSS13．0统计软件进行统计学分析；组间比较用方差分析，P〈0．05为差异具有统计学意义。结果与糖尿病假手术组和非糖尿病心肌梗死组相比，安慰剂组EF值显著降低[（32±3）％vs．（65±4）％，P〈0．01；（32±3）％vs．（38±4）％，P〈0．05]；E／A比值（P〈0．01）、左室非梗死区胶原容积分数（P〈0．05）及心室重量指数（P〈0．05）升高。与安慰剂组相比，雷米普利组EF值改善明显[（45±3）％vs．（32±3）％，P〈0．01]，且E／A比值显著降低（P〈0．01），左室非梗死区胶原容积分数下降、心室重量指数减少[（3．1±0．2）vs．（4．2±0．4），P〈0．01]。安慰剂组GLUT4mRNA及蛋白表达较非糖尿病心肌梗死组低，与糖尿病假手术组相比差异无统计学意义。与安慰剂组相比，雷米普利组GLUT4mRNA及蛋白表达升高。结论雷米普利可防治心室重构并改善心脏收缩及舒张功能；2型糖尿病合并心肌梗死大鼠心肌GLUT4mRNA和蛋白的表达下降，雷米普利可以改善糖尿病合并心肌梗死大鼠心肌GObjective To investigate ventricular remodeling and glucose transporter 4（GLUT4） expression in rat with type 2 diabetes mellitus （DM） complicated with myocardial infarction （MI）, as well as the effects of ramipril on ventricular remodeling and GLUT4 expression. Method Diabetic animal model （DM） was produced by cholesterol-diet and intraperitoneal injedtion of streptozotocin （STZ, 30 mg/kg）. The successful model was iden- tiffed based on the blood gulcose value 〉 6.7 mmol/L. Left anterior descending coronary artery was ligated to produce the model of MI. 46 wistar rats were randomly（random number） divided into 5 groups： DM ＋ MI ＋ placebo group（placebo group, n = 9）, DM ＋ MI ＋ ramipril group（ramipril group, n = 10）, DM ＋ sham-operation group （DM group, n = 10） ,non-DM group ＋ MI （MI group, n = 9）, and non-DM ＋ sham-operation group （ n = 8）. After six weeks, echocardiographic studies was performed ; Left ventricular mass index （LYMI）, myocardial infarct size and myocardial collagen volume fraction（CVF） of non-infarction area were also detected. GLUT4 mRNA in the myocardium away from infarction region was measured with real-time fluorescent quantitative RT-PCR and the expression of GLUT4 protein was measured with western blot. Analysis of variance was used to compare the groups. P -values 〈 0.05 were considered to indicate statistically significant differences. Results Compared with MI group and DM group,EF[（32±3）% vs.（65±4）%, P 〈0.01 and （32±3）% vs.（38±4）%, P 〈0.05 respectively] significantly decreased,while E/A ratio （P 〈 0.01）, LVMI （P 〈 0.05） and CVF （P 〈 0.05） obviously increased in placebo group. The expression of GLUT4 mRNA and protein were similar between placebo group and DM group, however, much lower than those in/VII group（P〈 0.01）. In rampiril group, E/A ratio （P 〈 0.01 ）, LVMI（3.1± 0.2 vs.4.2 ±0.4, P 〈 0.01 ） and CVF （ P 〈 0.05） significantly decreased compared with

关 键 词：大鼠 心肌梗死 2型糖尿病 雷米普利 心室重构 心脏彩色多普勒 心脏功能 葡萄糖转运体 

分 类 号：R587.1[医药卫生—内分泌] R542.22[医药卫生—内科学]