传染性法氏囊病病毒超强毒株HLJ-0504全基因组克隆及其新特征分析  

Cloning and sequence analysis of full-length genome of very virulent infectious bursal disease virus HLJ-0504

作　　者：高立[1] 祁小乐[1] 高玉龙[1] 高宏雷[1] 秦立廷[1] 邓小芸[1] 宇文延青[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2010年第5期401-404,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家973项目(2005CB523202);中国博士后科学基金(20080440921);黑龙江省博士后资助经费(LBH-Z08016)

摘　　要：为了解传染性法氏囊病病毒(IBDV)HLJ-0504株的分子生物学特征,本研究利用融合PCR技术获得HLJ-0504株全基因组序列。核苷酸和推导氨基酸序列遗传演化分析发现,HLJ-0504A节段位于IBDV超强毒的分枝上,而B节段则介于超强毒株和减毒株之间,形成一个独立分枝,属于一株新的自然重组病毒。VP2抗原性预测分析表明,HLJ-0504株VP2第Ⅰ亲水区中的氨基酸发生突变(D212N),该突变可能导致了HLJ-0504株抗原性发生漂变。本实验结果为深入研究IBDV的分子特征奠定了基础。The full length genomes of very virulent infectious bursal disease virus（vvIBDV） HLJ-0504 isolate was cloned by fusion PCR and the DNA fragments of segment A and B were sequenced.Genetic evolution analysis showed that the segment A of the HLJ-0504 isolate was located on the branch of vvIBDV,however the segment B formed an independent branch between attenuated and vvIBDV strains,indicating that natural reassortment might have occurred between different IBDV stains.There was an amino acid change from D to N at position 212 in the first hydrophilic region of VP2,which could result in the drift of the antigenicity of HLJ-0504.

关 键 词：传染性法氏囊病病毒超强毒 HLJ-0504株 全基因组 重组 

分 类 号：S852.65[农业科学—基础兽医学]