持续静脉输注替罗非班对动脉硬化兔血浆炎症因子的影响  

The Effect of Continuous Intravenous Drip Tirofiban on the Plasma Inflammatory Factors of Rabbit with Atherosclerosis

作　　者：谢双锋[1] 尹松梅[1] 李益清[1] 聂大年[1] 肖洁[1] 马丽萍[1] 王秀菊[1] 王景峰[2] 

机构地区：[1]中山大学附属第二医院血液内科,广东省广州市510120 [2]中山大学附属第二医院心内科,广东省广州市510120

出　　处：《中国动脉硬化杂志》2010年第2期105-108,共4页Chinese Journal of Arteriosclerosis

基　　金：广东省医学科研基金项目(B2007056)

摘　　要：目的探讨血小板膜糖蛋白Ⅱb/Ⅲa受体拮抗剂替罗非班在体内持续应用对动脉硬化兔血浆炎症因子的影响,为动脉硬化时合理应用血小板膜糖蛋白Ⅱb/Ⅲa受体拮抗剂提供理论依据。方法高脂饲料喂养新西兰大白兔12周形成动脉硬化模型后,分为4组,每组8只,分别恒速静脉滴注生理盐水、3.125、12.5 mg/L和50mg/L的替罗非班注射液,于注射0、12、24 h和48 h时,采用比浊法检测血小板最大聚集率和酶联免疫吸附法检测血浆P选择素、白细胞介素6、白细胞介素1β、肿瘤坏死因子α等炎症因子的水平,比较相关指标的变化。结果实验12、24 h和48 h时,随着替罗非班用药剂量增加,血小板最大聚集率逐渐减低(PANOVA<0.05),P选择素、白细胞介素1β、白细胞介素6、肿瘤坏死因子α逐渐减低(PANOVA<0.05)。对照组和3.125 mg/L替罗非班组12、24 h和48 h的血小板最大聚集率和0 h比较无改变(P>0.05);≥12.5 mg/L替罗非班用药后的血小板最大聚集率较用药0 h明显减低(P<0.05),P选择素、白细胞介素1β、白细胞介素6、肿瘤坏死因子α较0 h明显减低(P<0.05)。结论小剂量替罗非班并不改变血小板聚集,中剂量至大剂量静脉滴注时,血小板聚集和血清炎症因子明显抑制。Aim To explore the glycoprotein Ⅱb/Ⅲa receptor antagonist,tirofiban,on the plasma inflammatory factors of rabbits in vivo,and find some theoretical basis for appropriately using the glycoprotein Ⅱb/Ⅲa receptor antagonists in atherosclerosis.Methods New Zealand rabbits were fed with hyper-cholesterol forage for 12 weeks to form atherosclerosis.The rabbits were divided to 4 groups（n=8）,and had intravenous drip natural saline,3.125 mg/L,12.5 mg/L or 50 mg/L tirofiban in different group respectively.After the intravenous drip started for 0 h,12 h,24 h and 48 h,the platelet maximal aggregating rates [PAG（M）] were detected using turbidity method,the interleukin-6,interleukin-1β and tumor necrosis factor-α（TNF-α） level in the plasma were detected using enzyme linked immunosorbent assay（ELISA）.Parameters differences among 4 groups were compared.Results After intravenous dripping started for 12 h,24 h and 48 h,PAG（M） was reduced（PANOVA0.05）,P-selectin,interleukin-6,interleukin-1β and TNF-α were reduced（PANOVA0.05） along with the tirofiban concentration increase.In control group and 3.125mg/L tirofiban group,PAG（M） had no difference after intravenous dripping started for 12 h,24 h and 48 h（P0.05）.PAG（M）,P-selectin,interleukin-6,interleukin-1β and TNF-α were reduced significantly（P0.05） when ≥12.5 mg/L tirofiban was intravenous dripped.Conclusion The PAG（M） and inflammatory factors were not affected when tirofiban was intravenous dripped in small dosage.When the tirofiban was intravenous dripped in medium or large dosage,the PAG（M） and inflammatory factors were inhibited significantly.

关 键 词：血小板膜糖蛋白Ⅱb/Ⅲa受体拮抗剂 动脉硬化 炎症因子 

分 类 号：R311[医药卫生—基础医学]