真菌激发子对桦褐孔菌多酚积累的影响  

Effects of fungal elicitor on production of phenolic compounds by Phaeoporus obliquus

作　　者：赵艳霞[1] 缪康杰[1] 张梅梅[1] 郑维发[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,徐州221116

出　　处：《菌物学报》2010年第3期437-441,共5页Mycosystema

基　　金：江苏省自然科学基金(No.BK2009084);徐州师范大学国家自然科学基金预研究项目(No.08XLY14)

摘　　要：作者旨在阐明真菌激发子对桦褐孔菌多酚积累的影响。以摇瓶法培养桦褐孔菌,在其培养液中加入真菌激发子和一氧化氮合酶抑制剂氨基胍,观察桦褐孔菌多酚和一氧化氮的积累并测定菌丝体内一氧化氮合酶和苯丙氨酸解氨酶的活性。多酚以Folin-Ciocalteu法测定,一氧化氮的积累量用硝酸还原酶法测定,一氧化氮合酶和苯丙氨酸解氨酶活性均以分光光度计法测定。结果表明,添加45μg/mL的激发子可使桦褐孔菌菌丝体多酚的积累量达到46.5mg/g,显著地高于正常培养的菌丝体多酚积累量34.6mg/g;同时加入45μg/mL的激发子和10mmol/L氨基胍则使菌丝体多酚积累的最高水平降为34.8mg/g。此外,激发子的加入显著促进了一氧化氮的产生并提高了苯丙氨酸解氨酶活性,而这种促进和提升作用为氨基胍所抑制。这表明真菌激发子能显著地提升桦褐孔菌多酚类化合物的积累,而一氧化氮可能是这种提升作用的信号传导分子。This study was designed to evaluate the effects of fungal elicitor on the production of phenolic compounds in submerged cultures of Phaeoporus obliquus. Fungal elicitor and amino guanidine were supplemented to the cultures to determine the changes in accumulation of phenolic compounds and nitric oxide, the activities of nitric oxide synthase and phenylalanine ammonium-lyase. Phenol levels were measured using Folin-Ciocalteu method. Nitric oxide was measured by nitrate reductase method. The activities of nitric oxide synthase and phenylalanine ammonium-lyase were detected by spectrophotometric methods. The exposure of P. obliquus to fungal elicitor at a final concentration of 45μg/mL resulted in a highest production of phenolic compounds, with the level reaching 46.5mg/g, obviously higher than that in control culture; exposure to 45μg/mL of fungal elicitor together with 10mmol/L of amino guanidine led to a reduced production of phenolic compounds with a peak level reaching 34.8mg/g. In addition, the supply of fungal elicitor also promoted the production of nitric oxide and the activity of phenylalanine ammonia-lyase. This promotion, however, wasinhibited by NOS inhibitor amino guanidine. These suggest that up-regulation on accumulation of phenolic compounds in submerged cultures of P. obliquus by fungal elicitor is probably mediated by nitric oxide.

关 键 词：深层培养 一氧化氮 一氧化氮合酶 苯丙氨酸解氨酶 

分 类 号：S567.39[农业科学—中草药栽培]