低剂量高强度聚焦超声辐照兔肝后的病理转归  

Pathological regression of rabbit liver after low-dose irradiation with high intensity focused ultrasound

作　　者：张奕[1] 邹建中[1] 欧霞[1] 邹海蓉[1] 王雁[1] 

机构地区：[1]重庆医科大学生物医学工程系超声医学工程重庆市市级重点实验室,重庆400016

出　　处：《中国医学影像技术》2010年第4期620-622,共3页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金重点项目(30830040)

摘　　要：目的观察兔肝组织经低剂量高强度聚焦超声(HIFU)辐照后的病理转归,探讨低剂量HIFU辐照改变兔肝组织声环境的作用及机制。方法对10只新西兰大白兔分别于低剂量HIFU辐照其肝脏后即刻(第1天)、2、3、5及7天取材进行光镜和电镜观察。结果光镜下组织学变化:辐照后即刻,可见肝细胞水肿;第2、3天可见淤血,水肿加重;随后肝细胞逐渐恢复正常。电镜下超微结构变化:辐照后即刻,可见肝血窦内皮细胞脱落,肝细胞胞浆内细胞器肿胀;第2、3天可见肝血窦内红细胞聚集,肝细胞胞浆内出现空泡;随后细胞器肿胀逐渐减轻,直至恢复正常。结论低剂量HIFU辐照可使兔肝组织发生相应的变化,从而改变组织声环境。Objective To observe the pathological regression of rabbit liver after the low-dose irradiation with high intensity focused ultrasound （HIFU）,and to explore the role and mechanism of the irradiation in changing acoustic environment of rabbit liver. Methods Pathological study was performed in 10 New Zealand white rabbits,the livers were observed under light and electron microscope. The observation was done immediately after low-dose HIFU irradiation and in 2,3,5,7 days after the low-dose irradiation with HIFU. Results Light microscope changes：Edema existed in hepatocytes after irradiation instantly,congestion and aggravated edema were found at 2-3 days,then the injured cells recovered gradually. Electron microscope changes：Detached endothelial cells of hepatic sinusoid and swelling organelles were observed after irradiation,while 2 or 3 days later,erythrocyte aggregation was found in hepatic sinusoid and cavitation was found in cytoplasm. Thereafter,organelles swelling reduced till resumed to normal. Conclusion Low-dose HIFU irradiation can make corresponding changes to the acoustic environment of rabbit liver.

关 键 词：高强度聚焦超声 低剂量 组织声环境 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]