纳米金型压电石英DNA传感器的电化学阻抗谱研究  

Characterization of Nanogold-based Quartz Crystal Microbalance DNA Biosensor Array Using Electrochemical Impedance Spectroscopy

作　　者：夏涵[1] 王丰[1] 黄庆[1] 府伟灵[1] 黄君富[1] 

机构地区：[1]第三军医大学第一附属医院检验科,重庆400038

出　　处：《中华医院感染学杂志》2010年第11期1520-1523,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30672004);军队"十一五"课题(06G073);国家863计划子课题(2007AA022005)

摘　　要：目的采用电化学阻抗谱法表征所构建的纳米金型压电石英DNA传感器。方法采用构建的三电极系统,分别于空白石英晶振、探针固定后、6-巯基己醇封闭后、靶序列杂交后交流阻抗谱、纳米金磁微粒信号放大后对石英晶振表面进行交流阻抗谱分析,同时观察传感器的频率响应情况。结果进行纳米金磁微粒信号放大后,频率变化值为(127.5±6.8)Hz,与靶序列杂交、阴性对照和空白对照频率变化值差异有统计学意义(P<0.01),构建的三电极系统可成功检测石英晶振表面电化学变化,并且在检测过程中的各步骤,电子转移阻抗Ret均发生了明显增加(P<0.01),压电频率变化趋势与电化学阻抗变化趋势一致。结论电化学阻抗谱法可无损性地分析晶振表面膜层变化,验证了建立的压电石英DNA传感器检测方法和纳米金信号放大方法的真实性和有效性。OBJECTIVE To Characterize nanogold-based quartz crystal microhalance （QCM） DNA biosensor array using electrochemical impedance spectroscopy（EIS）. METHODS Faradaic impedance spectra measurements were performed as following： the bare electrode, after thiolated probe modification, after 6-mereaptohexanol modification, after the hybridization with target DNA and after the signal amplification with nanoparticles. In addition, the frequency shifts were also recorded. RESULTS The frequency shift caused by the conjugation of Au nanoparticles to target DNA （127.5 ± 6.8Hz） was significantly larger than that caused by the hybridization, the blank control and the negative control （P〈 0. 01）. The interracial electron-transfer resistances significantly increased after each step （P〈0.01）. CONCLUSIONS The EIS is proved to be a non-damage and useful technique for characterizing the multilayer films on the quartz crystal. The results well demonstrate the fabrication and detection process on the quartz crystal.

关 键 词：石英晶体微天平 传感器 电化学阻抗谱 

分 类 号：R446[医药卫生—诊断学]