艾滋病合并马尔尼菲青霉菌感染的实验室检测  

Laboratory Detection of Penicillium marneffei Infection Associated with AIDS

作　　者：于军校[1] 徐艳[2] 罗阳[1,3] 府伟灵[1] 钟白玉[2] 张晓兵[1] 

机构地区：[1]第三军医大学第一附属医院检验科,重庆400038 [2]第三军医大学第一附属医院皮肤性病科,重庆400038 [3]福建省医疗器械和医药技术重点实验室,福建福州350002

出　　处：《中华医院感染学杂志》2010年第11期1636-1638,共3页Chinese Journal of Nosocomiology

基　　金：国家"863"项目(2007AA02Z416);国家自然科学基金(30900348);全军科技成果推广扩试项目(08JKS01);军队科技攻关(08G089);重庆市科技攻关项目(CSTC;2008AC0001);重庆市自然科学基金(CSTC;2007BB5067);第三军医大学军事医学专项(2008XG24);福建省医疗器械和医药技术重点实验室开放基金(09001)

摘　　要：目的明确从AIDS患者血液、骨髓中检出的病原体为马尔尼菲青霉菌。方法取患者血液、骨髓于血培养瓶中置Bacter/Alert3D血培养仪中培养,待血培养仪报阳性即作革兰染色,并转种至马铃薯斜面培养基于27℃和37℃孵育,观察真菌生长情况及菌落形态,并作乳酸棉兰染色于显微镜下观察菌体特征。结果该菌为一双相真菌,27℃见菌丝相,呈淡黄色绒毛样,并产生酒红色色素,镜下可见典型的帚状枝及孢子链;37℃见酵母相,不产生特征性色素,镜下见酵母样细胞。结论从体内分离出马尔尼菲青霉菌,即可诊断为马尔青霉菌病,也可排除组织胞浆菌感染。OBJECTIVE To detect the Penicillium marneffei （PM） from the blood and bone marrow in a patient with AIDS. METHODS The blood or bone marrow was inoculated in the culture flask and inseted the hemoeulture meter of Baeter/Alert 3D. If it was positive then to make Gram staining,and inoculate on poteto dextrose agar at 25 ℃ and 37 ℃, respectively. The growth and the fungal colony of PM were inspected and the characteristics of PM by stain of lacto-phenol cotton blue were observed under microscope. RESULTS The fungus was a dimorphic, it appeared to be a filamentous type colony with light yellow villi,and produced wine color pigment in agar, and present typical bloom-like twigs and spore-chains under microscope. It appeared to be yeast phase and without characteristic pigment. It showed yeast-like cells under microscope. CONCLUSIONS The diagnositic standard of PSM depends on isolation of PM from patients, with exclusion of Histoplasma infection.

关 键 词：艾滋病 马尔尼菲青霉 马尔尼菲青霉病 

分 类 号：R512.91[医药卫生—内科学]