烟酸生物转化产物的双波长检测研究  

DETERMINATION OF NICOTINIC ACID BIOTRANSFORMED MIXTURE BY DUAL WAVELENGTH ULTRAVIOLENT SPECTROPHOTOMETRY

作　　者：尹祖建[1] 罗晖[2] 常雁红[1] 苏厚波[2] 肖宝清[1] 

机构地区：[1]北京科技大学环境工程系,北京100083 [2]北京科技大学生物科学与技术系,北京100083

出　　处：《化学分析计量》2010年第3期55-57,共3页Chemical Analysis And Meterage

基　　金：国家重点基础发展计划(2007CB714304)

摘　　要：建立了一种利用双波长分光光度法测定烟酸酶法转化液中烟酸和6-羟基烟酸浓度的方法,选择的波长为266.5、300 nm。对烟酸生物转化产物的混合物进行分析,烟酸测定结果的标准标准偏差不大于1.30%(n=6),6-羟基烟酸测定结果的相对标准标准偏差不大于1.54%(n=6)。烟酸和6-羟基烟酸的加标回收率为95.1%～105.1%,检出限分别为0.9 mg/L和0.26 mg/L。该法与常规的HPLC方法相比,快速、准确、适于大量样品的测定。A method for determination of nicotinic acid and 6-hydroxynicotinic acid in enzymatic transformed mixture by double - wavelength UV spectrophotometry was developed, and the wavelength of 266.5 nm and 300 nm were selected. The relative standard deviation for nicotinic acid was less than 1.3% and 6-hydroxynicotinic acid was less than 1.54%. The recovery of nicotinic acid and 6-hydroxynicotinic acid was 95.1% - 105. I%. The detection limits for nicotinc and 6-hydroxynicotinic were 0.9 mg/L and 0.26 mg/L, respectively. The dual wavelength spectrophotometry is rapid, accurate and superior to conventional HPLC method, especially in bulk samples determination.

关 键 词：双波长分光光度法 烟酸 6-羟基烟酸 

分 类 号：TQ216[化学工程—有机化工]