阿奇霉素对哮喘大鼠气道平滑肌细胞生长的影响  

Effects of azithromycin on the growth of airway smooth muscle cells in asthmatic rats

作　　者：尹娟[1] 戴元荣[1] 徐慧[1] 颜孙舜[1] 曾潍贤[1] 

机构地区：[1]温州医学院附属第二医院呼吸科,325027

出　　处：《浙江医学》2010年第5期685-687,697,I0003,共5页Zhejiang Medical Journal

摘　　要：目的 探讨阿奇霉素对哮喘大鼠气道平滑肌细胞（ASMCs）增殖的影响.方法 将20只SD大鼠随机分为对照组和哮喘组（哮喘组制备大鼠哮喘模型后,随机分为不同剂量的阿奇霉素组）,采用CCK-8法测定不同剂量的阿奇霉素组对哮喘大鼠气道平滑肌细胞的药物毒性反应,并分别采用琼脂糖凝胶电泳检测法和激光共聚焦显微镜观察法观察阿奇霉素各组的细胞凋亡情况,同时采用划痕法检测各阿奇霉素组平滑肌细胞的迁移能力.结果 （1）各剂量阿奇霉素组哮喘大鼠气道平滑肌细胞的生长率分别为（72.71±13.16）%、（66.42±4.90）%、（64.92±2128）%、（51.45±2.83）%;（2）与对照组比较,阿奇霉素各组均可以检测到气道平滑肌细胞染色体DNA发生片段化降解;（3）阿奇霉素各组均可显示典型的细胞凋亡形态学特征;（4）划痕法结果显示：阿奇霉素组的迁移数目和距离均较10%FBS血清刺激组减少和缩短（均P〈0.01）.结论 阿奇霉素可抑制哮喘大鼠气道平滑肌细胞增殖、迁移,促进细胞凋亡.Objective To investigate the effects of azithromycin on the growth of airway smooth muscle cells （ASMCs） in asthmatic rats. Methods The asthma model was induced in rats; the primary culture of ASMCs of asthmatic rats was established and cells of passages 3-8 were used in experiments. Cytotoxicity was detected with CCK8 method,the degradation of ASMCs chromatin DNA were analyzed by agarose gel electrophoresis. The morphological changes was observed under laser confocal microscope with Annexin V/PI staining. The migration of ASMCs was detected by wound healing assay. Results The viability of ASMCs with azithromycin were（ 72.71 ±13.16 ）%, （ 66.42 ± 4.90 ）%, （ 64.92 ± 2.28 ）% and （ 51.45 ±2.83 ）% respectively. Compared to the control group, the ASMCs chromosome DNA of azithromycin group degraded in a pattern of discrete fragments. Laser confocal microscopy revealed typical morphologic characteristics of apoptosis in azithromycin group. The distance （221.53 ±29.26 μm） and number （173 ± 7） of migration in azithromycin group were significantly lower than those of 10% FBS/DMEM stimulation （173 ±7 and 411.43 ±31.26 μ m, P〈0.01 ）, and those of DMEM stimulation were also lower than those of 10% FBS/DMEM stimulation （ 110±7 and 222.75± 28.84 μ m, P〈0.01 ）. Conclusion Azithromycin can inhibit the proliferation and migration of ASMCs, and can induce apoptosis in asthmatic rats.

关 键 词：哮喘 气道平滑肌细胞 阿奇霉素 

分 类 号：R966[医药卫生—药理学]