肌肉特化锚定蛋白β在小鼠心室肌组织中的增龄性变化及其与肥大心室肌细胞的相关性  

Age-related changes of muscle A-kinase anchoring protein β in mouse myocytes and its relation with hypertrophy of cardiac ventricular myocytes

作　　者：侯磊[1] 魏毅东[1] 徐亚伟[1] 宋静[1] 王勇[1] 于学靖[1] 陈艳清[1] 胡大一[2] 

机构地区：[1]同济大学附属第十人民医院心内科,上海200072 [2]北京大学人民医院心内科

出　　处：《上海医学》2010年第5期452-454,I0002,共4页Shanghai Medical Journal

基　　金：国家自然科学基金(30800466)资助项目

摘　　要：目的研究肌肉特化锚定蛋白β(mAKAPβ)在小鼠心室肌组织中的增龄性变化及其与肥大心室肌细胞的相关性。方法分别提取不同出生时间(1、7、40、90d)的昆明小鼠的心室肌组织,常规提取RNA后,应用实时聚合酶链反应检测不同年龄阶段小鼠心肌组织mAKAPβ基因表达水平。使用白细胞干扰因子(LIF)处理新生小鼠心肌细胞(实验组),了解LIF对新生小鼠心肌细胞中mAKAPβ基因表达水平的影响。结果随着小鼠年龄的增长,心室肌组织mAKAPβ的mRNA表达水平逐渐升高,出生1、7、40、90d的小鼠心室肌组织的mAKAPβ mRNA相对表达量分别为1.00±0.00、1.52±0.06、1.81±0.14和2.20±0.18,各组间差异均有统计学意义(P值分别<0.01、0.05)。在LIF干预48h后,实验组的心肌细胞面积明显增大,达(3107.84±731.98)mm2,显著大于对照组的(2348.19±533.00)mm2(P<0.05)。同时,实验组的mAKAPβ mRNA相对表达量为1.62±0.02,显著高于对照组的1.00±0.00(P<0.01)。结论 mAKAPβ基因表达水平在小鼠心室肌组织中呈增龄性变化,随着小鼠年龄的增长,心肌组织mAKAPβ mRNA相对表达量明显升高。在肥大的心室肌细胞中,mAKAPβ基因表达水平较正常心肌细胞明显升高。Objective To investigate the age-related changes of muscle A-kinase anchoring protein （mAKAPβ） in mouse myocytes and its relation with hypertrophy of cardiac myocytes. Methods The cardiac myocyte was extracted from mice on day 1, day 7, day 40 and day 90 after birth. Expression of mAKAPβmRNA was examined by real-time PCR in mouse cardiac myocytes before and after treatment with leukocyte inhibition factor （LIF）. Results Expression of mAKAPβ increased with age in mouse myocytes; and the expression levels on day 1, day 7, day 40 and day 90 after birth were 1.00±0.00, 1.52±0.06, 1.81±0.14 and 2.20±0.18, respectively （P〈0.01 or 0.05）. After LIF treatment for 48 hours, the cardiac myocytes became hypertrophy compared to untreated group （area of myocyte： [3 107.84 ± 731. 98] vs. [2,348. 19 ± 533. 00], P〈0.05）. Meanwhile, mAKAPβ expression in the LIF treatment group was significantly higher than that in the control group （[1.00±0.00] vs. [1.62±0.02], P〈0.01）. Conclusion mAKAPβexpression in mouse ventricular myoctye increases with age, and the expression of mAKAPβ is obviously higher in hypertrophic cardiac ventrioular myoctyes compared with the normal ones.

关 键 词：肌肉特化锚定蛋白β基因 心肌肥大 白细胞干扰因子 

分 类 号：R541[医药卫生—心血管疾病]