微量热法研究黄连及其主要组分配伍的抑菌作用  

Investigation on the Antibacterial Activity of Coptis chinensis Franch and Its Components Compatibility by Microcalorimetry

作　　者：代春美[1,2,3] 王伽伯[1] 孔维军[1,2] 肖小河[1] 彭成[2] 赵艳玲[1] 金城[1] 

机构地区：[1]解放军第三0二医院全军中药研究所,北京100039 [2]成都中医药大学药学院,成都610000 [3]辽宁医学院基础学院,锦州121001

出　　处：《化学学报》2010年第10期936-940,共5页Acta Chimica Sinica

基　　金：973计划(No.2007CB512607)资助项目;国家自然科学基金(No.30500665)资助项目

摘　　要：基于微量热法,研究黄连、黄连的主要组分小檗碱、药根碱、巴马汀及其配伍模拟方的抑菌作用.以HPLC法测定黄连中小檗碱、药根碱和巴马汀的含量,并根据其含量比值配伍模拟方;微量热法测定黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢曲线,得出相应的热动力学参数,并进行对应分析.结果表明黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢均有不同程度的抑制作用,黄连作用最强,单体生物碱作用弱,配伍模拟方作用增强,但并未显现明显协同作用,黄连的抑菌作用可能为多种活性成分的综合作用.The antibacterial activity of Coptis chinensis Franch and its key components including berberine,jatrorrhizine,palmatine and compatibility imitation recipes were studied by microcalorimetry. The contents of Ber,Jat and Pal were detected by high-performance liquid chromatography （HPLC） method and the compatibility imitation recipes were prepared according to the content proportion of three kinds of alkaloid in the herb. The power-time curves of Bacillus shigae growth affected by Coptis chinensis Franch,Ber,Jat,Pal and these compatibility imitation recipes were monitored by microcalorimetry. Meanwhile,the bio-thermo-kinetics parameters obtained from these curves were analyzed using correspondence analysis. Coptis chinensis Franch,Ber,Jat,Pal and compatibility imitation recipes had different inhibitory effect on Bacillus shigae growth. The antibacterial effects of Coptis chinensis Franch and the compatibility imitation recipes were stronger than single alkaloids in this herb,and the compatibility imitation recipes did not showed ob-vious synergistic effect. It could be concluded that the antibacterial effect of Coptis chinensis Franch was due to the integrated action of its many components.

关 键 词：微量热法 黄连 抑菌作用 

分 类 号：R285[医药卫生—中药学]