基因疫苗干预对感染小鼠心肌组织病理及超微结构的影响  

MYOCARDIAL HISTOPATHOLOGY AND ULTRASTRUCTURE CHANGES OF MICE INFECTED BY COXSACKIE VIRUS GROUP B TYPE 3 AFTER PRETREATED WITH RECOMBINANT VACCINE

作　　者：羡鲜[1] 韩小艳[2] 孟丽[3] 赵春芳[4] 王永祥[1] 

机构地区：[1]河北医科大学基础医学院病原生物学教研室,河北石家庄050017 [2]河北北方学院微生物学教研室,河北张家口075000 [3]河北医科大学电镜实验中心,河北石家庄050017 [4]河北医科大学基础医学院组织胚胎学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2010年第5期501-505,共5页Journal of Hebei Medical University

摘　　要：目的探讨基因疫苗干预对柯萨奇病毒B组3型(coxsackie virus group B type 3,CVB3)感染后,小鼠心肌组织病理及细胞超微结构的变化及意义。方法将BALB/C小鼠随机分为3组,每组11只,用空质粒pcDNA3、基因疫苗pcDNA3/VP1和融合基因疫苗pcDNA3/MDC-L19-VP1分别免疫,共3次,每次间隔4周。末次免疫后第3周,每组随机取3只小鼠,腹腔注射含3倍半数致死量(3 half lethal dose,3LD50)的CVB3病毒液,感染后第7天将小鼠处死,用于检测血中病毒滴度,并取心脏组织进行病理观察和细胞超微结构观察。每组剩余8只小鼠,腹腔注射致死量病毒液,观察并记录动物存活情况。结果预防性接种重组基因疫苗后,CVB3感染的小鼠生存率pcDNA3/MDC-L19-VP1组为37.5%,pcDNA3/VP1组12.5%,pcDNA3组11 d内全部死亡,3组生存率比较差异有统计学意义(P<0.01);3组小鼠血中的病毒滴度依次降低,且差异有统计学意义(P<0.01);光镜观察显示pcDNA3/MDC-L19-VP1组小鼠心肌组织细胞未出现严重的水肿和炎细胞浸润,电镜显示心肌细胞未出现核周水肿,肌原纤维未见断裂溶解,线粒体、肌浆网等细胞器未见明显异常,仅有少量炎性细胞浸润。结论融合基因重组疫苗可以有效的预防CVB3感染对心肌的损伤。Objective To study myocardial histopathology and uhrastructure changes of mice infected by coxsackie virus group B type 3 （CVB3） after pretreated with recombinant vaccines. Methods BALB/C mice were divided randomly into 3 groups, with 11 mice in each group. The plasmids of pcDNA3, pcDNA3/VP1 and pcDNA3/MDC-L19-VP1 were inoculated respectively to mice for three times. Three weeks after the third immunization, 3 mice from each group were challenged with 3 LD50 CVB3 and were sacrificed at the 7th day,the titers of blood viruses were evaluated. Then the hearts were stained to observe the changes of myocardial histopathology and uhrastructure. 8 mice from each group were subjected to intraperitoneal （ i. p. ） challenge with fatal dose of CVB3 and the number of surviving animals was monitored up to 3 weeks post infection. Results After CVB3 challenge, the survival rates of peDNA3/MDC-L19-VP1 group,peDNA3/VP1 group were 37.5% and 12.5% ,respectively. The mice of pcDNA3 group were all dead within 11 days; the virus titers of mice immunized with pcDNA3/MDC-L19- VP1 were lower than that of mice immunized with other plasmids （P 〈 0. 01 ）. The histopathology observation of pcDNA3/MDC-L19-VPlgroup indicate that myocardial cells in mice does not appear serious edema and inflammatory cell infiltration; Transmission electron microscope showed that myocardial cells do not appear perinuclear edema, fracture dissolution of muscle fibrils, no obvious mitochondria and sarcoplasmic reticulum abnormalities, and only a small amount of inflammatory cell infiltration. Conclusion fusion gene recombinant vaccine can effectively prevent myocardial damage induced by CVB3 infection.

关 键 词：疫苗 合成 柯萨奇病毒感染 小鼠 

分 类 号：R512.5[医药卫生—内科学]