Synthesis and Spectral Characterization of Novel Sulfhydryl-reactive Benzimidazole Fluorescent Probes  

作　　者：LEI Ymg-jie OUYANG Jie ZHANG You-lai DING Mei 

机构地区：[1]Department of Chemistry ＆ Chemical Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第3期402-405,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.20771082);the Natural Science Foundation of Tianjin Municipality, China(No.09JCYBJC05800);the Educational Commission of Tianjin Municipality, China (No.20080509)

摘　　要：Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.Three sulfhydryl-reactive fluorescent probes, which contain 2-（1H-benzoimidazol-2-yl）-phenol and maleimide group, were synthesized and their structures were characterized by IR and ^1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.

关 键 词：Fluorescent probe 2-（2-Hydroxyphenyl） benzimidazole Sullhydryl-reactive 

分 类 号：TQ463.4[化学工程—制药化工] TQ655