生长分化因子15在心脏成纤维细胞中的表达和意义  

Expression and Significance of Growth Differentiation Factor-15 in Myocardial Fibroblast

作　　者：汪宇鹏[1] 于海奕[1] 徐明[1] 高炜[1] 

机构地区：[1]北京大学第三医院心内科分子心血管学教育部重点实验室,北京100191

出　　处：《中国医药导刊》2010年第4期633-635,共3页Chinese Journal of Medicinal Guide

基　　金：国家重点基础研究发展计划("973计划")资助项目(2007cb512100)

摘　　要：目的:探讨生长分化因子-15(GDF15)在心脏成纤维细胞中表达及意义。方法:分离原代大鼠成纤维细胞,分为对照组,ET-1刺激组(10^(-6)mol/L),GDF15刺激组(3 pmol/L);利用realtime-PCR法检测GDF15 mRNA表达,用Western印迹法、ELISA法检测GDF15蛋白表达;用MTT和流式细胞术检测GDF15对成纤维细胞增殖的影响。结果:心脏成纤维细胞生理条件下表达GDF15,ET-1诱导GDF15表达增加,ET-1组GDF 15 mRNA是对照组(1.20±0.03)倍(P=0.02),上清中分泌的蛋白则是(3.27±0.81)pg/mL,高于对照组(0.41±0.17)pg/mL (P=0.013)。外源性给予GDF15刺激后,MTT测得OD值与对照组比较为(0.62±0.03)比(0.48±0.03),(P=0.029);而流式细胞术检测S期比例较对照组升高近3倍(9.62±1.17)%比(3.80±0.78)%,P=0.008),均提示能够促进成纤维细胞增殖。结论:GDF15在成纤维细胞中有表达并具有促进成纤维细胞增殖作用,提示其可能参与心肌纤维化的病理过程。Objective：To investigate the expression of Growth differentiation factor-15（GDFlS） and the effect of GDFI5 in the proliferation of fibroblasts.Methods：Primary myocardial fibroblasts were isolated from neonatal rats and divided into three groups treated with different conditions： ET-1（10^-6 mol/L）,GDF 15（3 pmol/L） and nothing.The expression of GDF 15 mRNA was detected by realtime-PCR and the expression of GDFI5 protein was shown by Western-Blot and Elisa.The proliferation of fibroblast induced by GDF-15 was measured by MTT and Flow cytometry. Results：GDF15 is upregulated in ET-1 group：the GDF15 mRNA is 1.20±0.03 fold of control group （P=0.02）, and protein in the supematant is （3.27±0.81）pg/mL vs （0.41±0.17）pg/mL（P=0.013）.GDF15 could enhance the MTT OD value from 0.48±0.03 to 0,62±0.03 （P=0.029）,it could raise the cell in S phase nearly 3 times more than control group （9.62±1.17）% vs （3.80±0.78）%,P=0.008）. Conclusinn：GDF15 is uprcgulatvd by ET-1 in myocardial fibroblasts.and it could enhance the proliferation of fibroblast,which may participate the progression of myocardial fbrosis.

关 键 词：生长分化因子15 成纤维细胞 内皮素1 心肌纤维化 

分 类 号：R541[医药卫生—心血管疾病]