HLA-G蛋白及基因在子宫腺肌病中的表达  

Expression of HLA-G Protein and Gene in Adenomyosis

作　　者：张涛[1] 杨一华[2] 刘建华[1] 孙桦[1] 陈鸣[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院妇产科,上海200011 [2]香港中文大学附属威尔士医院妇产科

出　　处：《现代生物医学进展》2010年第9期1635-1638,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨人类白细胞抗原G(human leucocyte antigen-G,HLA-G)在子宫腺肌病(adenomyosis,AM)患者在位、异位内膜中的表达及其生物意义,为AM的病因研究和治疗展望提供依据。方法:采用免疫组化和RT-PCR法检测36例AM患者和30例正常子宫内膜在位、异位内膜HLA-G蛋白及基因表达情况。结果:AM在位、异位内膜HLA-G蛋白及基因表达量显著高于正常子宫内膜(P<0.05);AM在位与异位内膜HLA-G蛋白表达量无显著差异(P>0.05);AM在位、异位内膜腺体细胞HLA-G蛋白的表达量呈正相关(r=0.948,P<0.05);AM在位、异位子宫内膜间质细胞HLA-G蛋白的表达量也呈正相关(r=0.863,P<0.05)。结论:HLA-G在AM在位、异位内膜中呈高表达,参与了AM的发病机制,HLA-G阳性的子宫内膜更容易逃避免疫细胞的攻击而浸润肌层生长。Objective：To investigate the expression of human leucocyte antigen-G（HLA-G） in the eutopic and ectopic endometrium of adenomyosis（AM） for the immunological pathogenesis of AM.Methods：Immunohistochemical staining and nested RT-PCR were used to examine HLA-G protein and mRNA expression in eutopic and ectopic endometrium of thirty-six patients with AM and thirty normal women.Results：HLA-G protein and DNA were expressed significantly higher in eutopic and ectopic endometrium of AM than normal endometrium（P0.05）;There was no obvious difference of HLA-G expression between eutopic and ectopic endometrium of AM（P0.05）;HLA-G expression in glandular cells of eutopic endometrium was positively correlated with that in glandular cells of ectopic endometrium（r=0.948,P0.05）;HLA-G expression in stromal cells of eutopic endometrium was positively correlated with that in stromal cells of ectopic endometrium（r=0.863,P0.05）.Conclusion：HLA-G is highly expressed in eutopic and ectopic endometrium of AM,which may play certain role in the pathogenesis.Endometrium expressing HLA-G is more easily to escape from immune system destruction and successfully implant and grow in myometrium.

关 键 词：HLA-G 子宫腺肌病 在位内膜 异位内膜 免疫逃逸 

分 类 号：R711.71[医药卫生—妇产科学]