肝纤维化形成过程中RECK基因表达和MMP-2活性的相关性研究  

Correlative research between RECK expression and matrix metalloproteinase-2 activity in the formation process of hepatic fibrosis

作　　者：郭珊珊[1] 陈平圣[1] 李静[1] 吴楠[1] 刘东风[1] 

机构地区：[1]东南大学医学院病理学与病理生理学系,江苏南京210009

出　　处：《现代生物医学进展》2010年第9期1642-1645,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30470780)

摘　　要：目的:研究肝纤维化形成过程中RECK基因的表达与基质金属蛋白酶-2(MMP-2)活性之间的相关性。方法:SD雄性成年大鼠(25只)随机分为对照组(5只)和肝纤维化组(20只)。对照组每周2次纯花生油皮下注射,剂量为3ml·kg-1,第8周全部处死。肝纤维化组每周2次用50%CCl4油溶液皮下注射,剂量为3ml·kg-1,建立起肝纤维化模型,并分别于第1、2、4、6、8周末各处死4只动物,留取肝组织备用。天狼猩红染色判断肝纤维化程度,RT-PCR法检测RECK基因的表达情况,明胶酶谱法检测肝组织MMP-2的活性。结果:MMP-2的活性随肝纤维化的形成而逐渐升高,而RECK基因表达的变化与之相反,RECK的表达和MMP-2的活性呈负相关(P<0.05)。结论:在大鼠肝纤维化形成过程中,RECK的表达与MMP-2活性可能有密切关系,前者对后者可能起抑制作用。Objective：To investigate the relationship between RECK expression and matrix metalloproteinase-2（MMP-2） activity in the formation process of hepatic fibrosis in rats.Methods：Spraque Dawley male rats（25 rats） were randomly divided into two groups：control group（5 rats） and hepatic fibrosis group（20 rats）.The control group were injected hypodermically by oil solution twice a week（the dosage is 3 ml·kg-1） and all of them were sacrificed at the end of eighth week.The hepatic fibrosis model was induced by 50 % CCl4 in oil solution twice a week（the dosage is 3 ml·kg-1） in the experiment group and four rats were randomly sacrificed at the end of the first,second,fourth,sixth,eighth week respectively.The collagen deposition,RECK expression and MMP-2 activity in the liver tissues were detected by Sirius red staining,RT-PCR and zymography respectively.Results：It was found that MMP-2 activity increased gradually in the formation process of the hepatic fibrosis.But RECK expression had reversed change at the same time.There was negative correlation between RECK expression and MMP-2 activity in the liver tissues（P0.05）.Conclusions：The results suggest that MMP-2 activity and RECK expression may be closely related in the formation process of hepatic fibrosis.RECK maybe have depressant effect on MMP-2 activity.

关 键 词：RECK基因 基质金属蛋白酶-2 肝纤维化 大鼠 

分 类 号：Q953[生物学—动物学] Q78