卡托普利对高血压大鼠动脉血管平滑肌细胞内质网相关因子葡萄糖调节蛋白78和C/EBP同源蛋白表达的影响  

Effect of Captopril on GRP78 and CHOP Expression of Vascular Smooth Muscle Cells in Hypertension Rats

作　　者：黄金燕[1] 赵连友[1] 郑强荪[1] 林可[1] 丁璐[1] 艾永飞[1] 杨竞霄[1] 

机构地区：[1]第四军医大学唐都医院心血管内科,陕西西安710038

出　　处：《现代生物医学进展》2010年第9期1680-1683,1689,共5页Progress in Modern Biomedicine

基　　金：陕西省科学技术攻关课题(2005K13-G1-3)

摘　　要：目的:研究卡托普利对早期高血压大鼠动脉血管平滑肌细胞内质网相关因子葡萄糖调节蛋白78(glucose-regulated protein of 78 kd,GRP78)和C/EBP同源蛋白(CAAT/enhancer binding protein homologous protein,CHOP)表达的影响。方法:将18只成年雄性Sprague-Dawley(SD)大鼠随机分为对照组、模型组和卡托普利组(n=6),模型组和卡托普利组均采用大鼠腹主动脉结扎建立高血压大鼠模型。4周后测量血压,应用免疫组化方法检测主动脉血管平滑肌细胞GRP78和CHOP的表达;缺口末端标记法(TUNEL)检测细胞凋亡。结果:(1)模型组平均动脉压(mean arterial blood pressure,MAP)明显增加,卡托普利组血压低于模型组,高于对照组,差异具有统计学意义(P<0.05);(2)模型组内质网因子GRP78、CHOP表达均增加,卡托普利组GRP78、CHOP表达低于模型组,高于对照组,差异具有统计学意义(P<0.01);(3)模型组血管平滑肌细胞凋亡率减少,卡托普利组血管平滑肌细胞凋亡率高于模型组,低于对照组,差异具有统计学意义(P<0.01)。结论:卡托普利可降低高血压大鼠动脉血管平滑肌细胞GRP78和CHOP的表达,增加细胞凋亡。可能与其减弱高血压所致内质网反应,维持血管平滑肌细胞的增殖/凋亡平衡有关。Objective：To study the effect of captopril to the expression of glucose-regulated protein of 78 kd（GRP78） and CAAT/enhancer binding protein homologous protein（CHOP） the correlation factors of endoplasmic reticulum in the vascular smooth muscle cells of early hypertension rats.Methods：Eighteen Sprague-Dawley（SD） rats were divided randomly into three groups：the control group（n=6）、the model group（n=6） and the captopril group（n=6）.The rats in the model group and the captopril group received abdominal transverse aortic constriction（TAC）.Four weeks later,blood pressure was measured,expression of GRP78 and CHOP in the vascular smooth muscle cells of each group was detected by immunohistochemistry,apoptosis was detected by TdT-mediated dUTP nick end labeling（TUNEL）.Results：①Mean arterial blood pressure（MAP） of the model group increased significantly（P0.05）,compared with that of the control group.MAP of the captopril group was higher than the control group,and lower than the model group,and the difference was significant（P0.05）.② The expression of GRP78 and CHOP of the model group increased significantly（P0.01）,compared with that of the control group.The expression of GRP78 and CHOP of the captopril group was higher than the control group,and lower than the model group,and the difference was significant（P0.01）.③Apoptosis rate of the vascular smooth muscle cells in the model group decreased significantly（P0.01）,compared with that of the control group.That of the captopril group was lower than the control group,and higher than the model group,and the difference was significant（P0.01）.Conclusions：Captopril reduced the the expression of GRP78 and CHOP in the vascular smooth muscle cells of hypertensive rats,increased apoptosis.That might relate to captopril＇s weakening the endoplasmic reticulum response which induced by hypertension,and maitaining of vascular smooth muscle cell proliferation /apoptosis balance.

关 键 词：高血压 卡托普利 内质网相关因子 葡萄糖调节蛋白78 C/EBP同源蛋白 血管平滑肌细胞 凋亡 

分 类 号：R541.3[医药卫生—心血管疾病]