幼年特发性关节炎患儿HLA-DRB1基因多态性分析  

HLA-DRB1 allelic polymorphism in children with juvenile idiopathic arthritis

作　　者：吴娟 曾华松[1,2] 

机构地区：[1]广州市妇女儿童医疗中心儿童院区 [2]广州医学院附属儿童医院过敏免疫风湿科,广东广州510120

出　　处：《中国当代儿科杂志》2010年第5期333-337,共5页Chinese Journal of Contemporary Pediatrics

基　　金：国家人事部留学归国人员启动基金(200499);广东省自然科学基金(2009);广东省科技厅科技攻关重点项目(2003B30503);广州市科技局科技攻关重大项目(2003Z2-E0181);广州市科技局科技攻关一般引导项目(2005Z1-E0104);广州市卫生局重点项目(2005-Zdi-08)

摘　　要：目的研究广东地区汉族幼年特发性关节炎(JIA)患儿HLA-DRB1位点等位基因多态性分布特点。方法采用序列特异性引物-聚合酶链反应(SSP-PCR)技术对94例JIA患儿和226例健康儿童HLA-DRB1的13个等位基因进行分析,并分析上述基因在各组中的分布。结果 JIA患儿HLA-DRB1*08(P=0.0014,OR=2.26)的分布频率明显高于健康儿童,HLA-DRB1*12(P=0.032,OR=0.55)的分布频率明显低于健康儿童。在JIA全身型及多关节型中HLA-DRB1*08明显增高,而HLA-DRB1*15在JIA全身型及少关节型中明显降低,观察组与对照组比较P<0.05,差异有统计学意义,其他各位点差异无统计学意义。结论 HLA-DRB1*08可能是JIA小儿的易感基因。HLA-DRB1*12可能是JIA小儿的保护基因,其中HLA-DRB1*08是全身型及多关节型的易感基因,而HLA-DRB1*15与全身型及少关节型保护基因密切相关。Objective To investigate the predisposing alleles of HLA-DRB1 genes in Han children with juvenile idiopathic arthritis（JIA） from Guangdong Province,China.Methods Polymerase chain reaction-specific sequence primers（PCR-SSP） method was used to type HLA-DRB1 subregions in 94 Han children with JIA and 226 Han healthy controls.Results The frequency of HLA-DRB1＊08 allele in the JIA group was significantly higher than that in the control group（P=0.0014,OR=2.26）,in contrast,the frequency of HLA-DRB1＊12 allele was significantly lower than that in the control group（P=0.032,OR=0.55）.It was found that in children with So-JIA subset（P=0.023,OR=2.25） and polyarthritis JIA subset（P=0.034,OR=2.81）,the allele HLA-DRB1＊08 was expressed most commonly.The allele HLA-DRB1＊15 was expressed in a lower frequency in children with So-JIA subset（P=0.049,OR=0.413） and oligoarthritis subset（P=0.045,OR=0.16） compared with that in the control group.Conclusions HLA-DRB1＊08 may be the susceptible allele of JIA,while HLA-DRB1＊12 may be the protective allele of JIA in Han children from Guangdong Province.HLA-DRB1＊08 is the susceptible allele of So-JIA and polyarthrits.HLA-DRB1＊15 is the protective allele for systemic JIA and oligoarthritis.

关 键 词：幼年特发性关节炎 HLA-DRB1 PCR-SSP 儿童 

分 类 号：R725.9[医药卫生—儿科]