增强紫外线B辐射对小麦根尖细胞微管骨架的影响  

Influence of Enhanced Ultraviolet-B Radiation on Microtubule Cytoskeleton in Wheat Root-tip Cells

作　　者：郭爱华[1] 高丽美[1] 李永锋[1] 翟菁如[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院细胞工程研究所,临汾041004

出　　处：《中国细胞生物学学报》2010年第2期256-260,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30671061);山西省自然科学基金(No.2008011059-1;No.20041101)资助项目~~

摘　　要：以小麦幼苗根尖为材料,采用间接免疫荧光标记技术并结合激光共聚焦扫描显微系统,研究了增强紫外线B(ultraviolet-B,UV-B)辐射(10.08 kJ·m^(-2)·d^(-1))对小麦根尖细胞微管骨架的影响,为进一步研究微管骨架与"分束分裂"的关系打下基础。研究表明,小麦根尖分裂过程中微管骨架排列呈现一定的周期性,对照组中微管周期结构明显清晰,荧光较强。而增强UV-B辐射处理的小麦根尖细胞中,其微管结构紊乱,周质微管骨架定向发生改变,或解聚呈片段和点状分布;出现两条早前期带的异常结构,纺锤体微管聚集,末期成膜体弥散或缺失。In order to further investigate the effects of ultraviolet-B （UV-B） radiation on the relationships between microtubule arrangements and ＇Partition-bundle division＇, the dynamic variation of microtubules in wheat root-tip cells exposed to enhanced UV-B radiation （10.08 kJ.m-2.d-1） was studied with confocal laser scanning microscopy （CLSM）. The results showed that the microtubule arrays presented a periodicity during mitosis of wheat root-tip cells, the obvious microtubule cycle and high fluorescence intensity were observed in control group; However, under the condition of enhanced UV-B radiation, the structure of microtubules were disorder while microtubules appeared a lot of abnormal arrangements. The cortical microtubules changed orientation to network and depolymerized significantly to stick and spot; the cells had abnormal structure of double preprophase bands; the arrangement of spindle microtubules showed accumulation and the phragmoplast microtubules were dispersivity or disappeared due to enhanced UV-B radiation.

关 键 词：微管骨架 增强UV—B辐射 小麦 激光共聚焦扫描显微镜 分束分裂 

分 类 号：S512.1[农业科学—作物学] Q942[生物学—植物学]