nPKC和cPKC调节骨骼肌葡萄糖转运体4型转位的研究  

Study on nPKC and cPKC Modulation of Glucose Transporter Type 4 Translocation in Skeletal Muscle Cells

作　　者：李金茹[1] 牛文彦[1] 初桂兰[2] 

机构地区：[1]天津医科大学基础医学院,300070 [2]天津医科大学总医院

出　　处：《天津医药》2010年第5期398-400,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30570912);国家自然科学基金委员会-加拿大卫生研究院健康研究合作项目(项目编号:30611120532);天津市科学技术委员会应用基础研究重点项目(项目编号:07JCZDJC07900);天津市科技支撑计划项目(项目编号:09ZCZDSF04500)

摘　　要：目的:探讨新颖型蛋白激酶C(nPKC)和传统型蛋白激酶C(cPKC)调节骨骼肌葡萄糖转运体4型(GLUT4)转位的作用。方法:C2C12GLUT4myc肌管分为Basal组、PMA组(PMA孵育)、Go6983+PMA组(Go6983预孵育,再与PMA共孵育)、Go6976+PMA组(Go6976预孵育,再与PMA共孵育)、PMA24h+PMA组(PMA预孵育24h后,PMA孵育)和PMA24h+Go6976+PMA组(PMA预孵育24h,Go6976预孵育后,Go6976和PMA共孵育),应用吸光度分析法测定细胞表面的GLUT4myc水平。结果:与Basal组相比,PMA组、Go6976+PMA组、PMA24h+PMA组细胞表面GLUT4myc转位明显增加,差异均有统计学意义(P<0.05),与PMA组相比,Go6983+PMA组、Go6976+PMA组、PMA24h+PMA组和PMA24h+Go6976+PMA组细胞表面GLUT4myc转位明显减少,差异均有统计学意义(P<0.05)。结论:PMA激活PKC,增强骨骼肌GLUT4转位;抑制nPKC或cPKC的活性可降低PMA刺激的骨骼肌GLUT4转位;激活nPKC或cPKC可调节骨骼肌GLUT4转位。Objective： To evaluate the effects of novel protein kinase C （nPKC） and conventional protein kinase C（cPKC） on modulation of glucose transporter 4 （GLUT4） translocation in skeletal muscle cells.Methods： C2C12GLUT4myc myotubes were divided into control group,PMA group （PMA incubation）,Go6983＋PMA group （Go6983 pre-incubation,Go6983 and PMA co-incubation）,Go6976＋PMA group （Go6976 pre-incubation,Go6976 and PMA co-incubation）,PMA24 h＋PMA group （PMA 24 h-preincubation,PMA incubation） and PMA24 h＋Go6976＋PMA group（PMA 24 h-preincubation,Go6976 preincubation,Go6976 and PMA co-incubation）.Levels of GLUT4myc were measured by absorbance.Results： Compared with control group,GLUT4myc translocation on cellular surface increased significantly in PMA group,Go6976 ＋PMA group and PMA24 h＋PMA group.Compared with PMA group,GLUT4myc translocation on cellular surface was significantly reduced in Go6983＋PMA group,Go6976＋PMA group,PMA24 h＋PMA group and PMA24 h＋Go6976＋PMA group.Conclusion： PMA activated PKC and increased GLUT4 translocation in skeletal muscle cells.GLUT4 translocation stimulated by PMA can be reduced by inhibited activity of cPKC and nPKC in skeletal muscle cells.Therefore,activation of nPKC and cPKC can modulate translocation of GLUT4 in skeletal muscle cells.

关 键 词：蛋白激酶C 葡萄糖转运体4型 肌 骨骼 血糖 转位 佛波醇酯类 血清 动物 

分 类 号：R587.1[医药卫生—内分泌]