正己烷慢性吸入致小鼠肺损伤Clara细胞分泌蛋白的表达及意义  

Expression and significance of Clara cell secretory protein in injury lungs of Kunming mice after n-hexane long-term inhalation

作　　者：张大勇[1,2] 黄中新[3] 蒋立娣[1] 楼雪芳[1] 姚雪燕[1] 

机构地区：[1]浙江大学城市学院,杭州310015 [2]上海复旦大学医学院 [3]暨南大学

出　　处：《中华劳动卫生职业病杂志》2010年第5期325-328,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的观察正己烷慢性吸入致昆明种小鼠肺损伤中Clara细胞分泌蛋白（clara cell secretory protein，CCSP）的表达情况，探讨Clara细胞在正己烷慢性吸入致肺损伤中的作用。方法24只昆明种小鼠随机分为4组（正常对照组及染毒4周、8周、12周组），初始正已烷染毒浓度为17．6g／m3，每天8h，每周染毒6d。染毒结束后即刻处死，气相色谱-质谱联用仪测全血中正己烷浓度。对肺组织进行HE染色，观察光学显微镜下肺组织形态学变化，免疫组织化学法观察细支气管内CCSP阳性反应的Clara细胞比例及肺组织内溶菌酶阳性反应的巨噬细胞数量变化。结果各染毒组小鼠血中正己烷平均浓度均明显高于对照组，差异均有统计学意义（P〈0．01）。各染毒组小鼠肺脏出现进行性的间质性炎症反应。染毒4周、8周和12周组Clara细胞在终末性细支气管和呼吸性细支气管上皮细胞中比例分别为（73．33±4．21）％、（60．98±4．94）％、（34．04±2．33）％和（75．44±．91）％、（58．54±4．86）％、（33．35±2．67）％，较对照组[（80．26±6．43）％和（81．74±7．75）％1明显下降，差异均有统计学意义（P〈0．05或P〈0．01），各染毒组肺组织内巨噬细胞数量分别为（21．39±7．41）、（28．54±10．73）、（48．97±19．55）个／视野，较对照组[（7．84±3．12）个，视野]明显增多，且随染毒时间延长，巨噬细胞数量呈增加的趋势，差异均有统计学意义（P〈0．05或P〈0．01）。结论慢性吸入正已烷可致昆明种小鼠细支气管上皮内Clara细胞持续受损，进而引起肺脏慢性炎症。Objective To observe the expression of Clara cell secretory protein （CCSP） in the Kunming mouse model of n-hexanc long-term inhalation, and to discuss the functions of Clara cell in injury lung induced by n-hexane. Methods 24 healthy mice were randomly divided into 4 groups： one control group and three n- hexanc groups （4 w, 8 w and 12 w）, 6 each group. Primary concentration of n-hexane was 17.6 g/m3, 8 hours per day, 6 d per week. After inhalation, n-hexane concentration of blood from celiac artery was detected. The lungs wcrc embedded with paraffin and HE staining in the routine. The ratio of Clara cells with CCSP reaction in bron- chiolc and the number of maerophage cells with lysozyme reaction were determined by immuno-histochemistry. Results In the poisoning groups, the average n-hexane concentration of blood was significantly higher than that of the control group （P〈0.01）. There were apparent pathologic damages in lungs of the poisoning mice. In poison- ing 4 w, 8 w and 12 w groups, the ratio of Clara cells was significantly decreased [（73.33 ±4.21 ）%, （60.98±4.94）%, （34.04±2.33）% in terminal bronehiole, and （75.44±7.91）%, （ 58.54±4.86）%, （33.35±2.67）% in respiratory bronehiole] as compared with the control mice [（80.26±6.43）% and （81.74±7.75）%, P〈0.05 or P〈 0.01], meanwhile the numbers of maerophage cells were gradually increased [（21.39±7.41 ）, （28.54±10.73）, （48.97±19.55） per microscopic field at 200x] in poisoning mice than those in control mice [（7.84±3.12） per microscopic field at 200x, P〈0.05 or P〈0.01]. Conclusion In injury lungs after n-hexane inhalation, Clara cells are the target cells of n-hexane toxicity effect. Clara cells play an extensive protective role in lung inflammation.

关 键 词：正己烷 肺损伤 CLARA细胞分泌蛋白 溶菌酶 

分 类 号：R686[医药卫生—骨科学]