子痫前期胎盘组织中miR-376c与miR-210的表达及其临床意义  

Expression of miR-376c and miR-210 in Human Placentas from Preeclamptic Pregnancies

作　　者：朱晓明[1] 尹国武[1] 王晓红[1] 杨华光[1] 姚元庆[1] 

机构地区：[1]第四军医大学唐都医院,陕西西安710038

出　　处：《实用妇产科杂志》2010年第5期380-382,共3页Journal of Practical Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(编号:30070787)

摘　　要：目的:检测miR-376c与miR-210在子痫前期患者与正常孕妇胎盘组织中的表达差异。方法:选取剖宫产分娩的产妇,其中轻度子痫前期患者8例(mPE组),重度子痫前期患者15例(sPE组),正常妊娠妇女11例(对照组),收集其胎盘组织。通过实时定量RT-PCR(realtimeRT-PCR)技术检测胎盘组织中miR-376c与miR-210的表达水平。结果:miR-376c与miR-210在子痫前期患者的胎盘组织中均存在异常表达。miR-376c在mPE与sPE组表达量均降低,分别为对照组表达水平的0.57倍(P<0.05)与0.50倍(P<0.05);miR-210在mPE组表达量降低,而在sPE组表达增高,分别为正常对照组表达水平的0.53倍(P<0.05)与2.91倍(P<0.05)。结论:miR-376c与miR-210的表达异常可能参与了子痫前期的发生和病理生理过程。Objective：The expression of miR-376c and miR-210 in placentas from preeclamptic pregnancies and normal pregnancy were investigated.Methods：Placentas were obtained during elective cesarean section from patients with mild preeclampsia（mPE,n=8）,severe preeclampsia（sPE,n=15）and normal pregnancy（control group,n=11）.The miR-376c and miR-210 expression were assessed by real time RT-PCR.Results：The expression of miR-376c and miR-210 were abnormal in the placentas of preeclamptic pregnancy.miR-376c was down regulated in mPE（0.57-fold）and sPE groups（0.50-fold）than control group.miR-210 was down regulated in mPE group（0.53-fold）and up regulated in sPE group（2.91-fold）compared with control group.Conclusions：The abnormal expression of miR-376c and miR-210 might involved in the generation and pathogenesis of preeclampsia.

关 键 词：微小核糖核酸 胎盘 子痫前期 

分 类 号：R714.24[医药卫生—妇产科学]