外源性视黄酸对人巩膜成纤维细胞MMP-2及TIMP-2 mRNA水平的影响  

Effects of exogenous retinoic acid on mRNA levels of MMP-2 and TIMP-2 in human scleral fibroblasts

作　　者：陈林华[1] 王教[1] 陈晓燕[1] 鹿润霞[1] 周翔天[1] 

机构地区：[1]温州医学院附属眼视光医院,浙江温州325027

出　　处：《中华眼视光学与视觉科学杂志》2010年第2期91-94,共4页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：国家自然科学基金重点资助项目（30830107）;国家自然科学基金资助项目（30973278）

摘　　要：目的 研究外源性视黄酸（RA）对人巩膜成纤维细胞（HSF）生长的调控,以及对基质金属蛋白酶2（MMP-2）和基质金属蛋白酶2组织抑制剂（TIMP-2）mRNA水平的影响.方法 体外培养HSF,取第5～7代细胞,经10^-10、10^-9、10^-8、10^-7、10^-6mol/L浓度的RA作用6 d后,进行细胞计数,观察RA对HSF生长的调控情况;用Real-time PCR检测RA作用后HSF中MMP-2、TIMP-2的mRNA水平.对不同浓度组问比较采用单因素方差分析,两两比较采用t检验.结果 RA作用HSF 6 d后,浓度≥10^-9 mol/L RA组对细胞的生长抑制作用差异均有统计学意义（P〈0.05）,随着RA浓度增高,细胞数量逐渐减少,抑制作用呈剂量效应.RA作用6 d后,RA≥10^-8 mol/L时,HSF中MMP-2 mRNA水平有升高趋势,但各组差异无统计学意义（P〉0.05）;RA≥10^-9 mol/L时,TIMP-2 mRNA水平下降（P〈0.05）.结论 RA能够抑制HSF的生长,并可能通过调控TIMP-2的mRNA水平,使巩膜主动重塑.Objective To study effects of exogenous retinoic acid （RA） on the growth of human scleral fibroblasts （HSF） and the mRNA level of matrix metalloproteinase-2 （MMP-2） and its tissue inhibitor （TIMP-2）. Methods HSF of 5-7 passages were quantitated after being treated with RA at concentrations of 10^-10 mol/L, 10^-9 mol/L,10^-8 mol/L, 10^-7 mol/L, 10^-6 mol/L, respectively for 6 days. The mRNA level of MMP-2 and TIMP-2 was measured by Real-time PCR followd by analysis using one-way ANOVA. Results The growth of HSF was significantly inhibited after 6 days of treatment with at least 10^-9 mol/L of RA （P〈0.05）. The reduction in number of HSF was positively correlated to the RA concentrations used. The mRNA level of MMP-2 was increased when the concentration of RA 〉 10^-8 mol/L. However, there was not significantly different between RA treated and non-RA treated HSF （P〉0.05）. The mRNA level of TIMP-2 decreased （P〈0.05） in HSF treated with RA at concentration ≥10^-9 mol/L. Conclusion RA could inhibit the growth of HSF cells and downregulate the mRNA level of TIMP-2, probably resulting in scleral remodeling.

关 键 词：维甲酸 巩膜 成纤维细胞 人 基质金属蛋白酶2 基质金属蛋白酶2组织抑制剂 

分 类 号：R285.5[医药卫生—中药学]