热处理后晶状体上皮细胞热休克蛋白27的表达及Quercetin对其的影响  

Expression of HSP27 in lens epithelial under high-temperature stress conditions and the influence from Quercetin

作　　者：陆飞[1] 邵彦[1] 马颖[1] 

机构地区：[1]大连医科大学附属第二医院眼科,辽宁大连116027

出　　处：《中华眼视光学与视觉科学杂志》2010年第2期110-112,共3页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：辽宁省自然科学基金资助项目（20072168）

摘　　要：目的 研究在高温的应激条件下,热休克蛋白27（HSP27）在大鼠晶状体上皮细胞（LEC）中不同时期的表达及Quercetin（HSP阻滞剂）对其的调节作用.方法 将60只SD大鼠随机分为3组：A组为空白对照组,B组为热处理组,C组为热处理加Quercetin组.所谓热处理为：将大鼠置于45℃水浴箱（恒温水）中使其肛温升至42℃后维持15 min,常温下放置2 h,以后隔日水浴一次.分别于试验1周、2周、3周、4周后脱臼致死各组5只大鼠并摘取眼球,取出晶状体,采用免疫组化技术检测HSP27在各组LEC中的表达情况.结果 每周各组之间LEC中HSP27表达的差异均有统计学意义（F分别为80.99、261.3、242.6、719.5,P均〈0.05）,B组表达明显高于A、C组,C组在第2周后表达明显高于A组;A组各个时间段的差异无统计学意义（F=0.086,P〉0.05）,B组则存在显著统计学意义（F=563.013,P〈0.05）,C组存在显著统计学意义（F=370.291,P〈0.05）.结论 热处理能促进晶状体上皮细胞中HSP27的表达,并随时间延长表达增强,从而可能对晶状体上皮具有保护作用.Quercetin对其有抑制作用,即使在Quercetin抑制作用下热处理仍能使晶状体上皮细胞中HSP27的表达明显增加.Objective To investigate the expression of heat shock protein 27 （HSP27） in rat lens epithelial cell （LEC） for different periods of time, and to investigate the regulatory role of Quercetin （HSP blocking agent） under high-temperature stress conditions. Methods Sixty SD rats were randomly divided into 3 groups： group A was the normal control group, group B was the thermotolerance treatment group, and group C was the thermotolerance treatment with Quercetin group. The heat treatment procedure was as follows： the SD rat was placed in a water bath box at 45℃ （permanent lukewarm water） causing its rectal temperature rise to 42℃ and maintain for 15 min. The rat was then removed and placed in a normal temperature environment for 2 h. After that, the rats were placed in the water bath once every other day. At 1 week, 2 weeks, 3 weeks, and 4 weeks, S rats in each group were sacrificed, the eyeballs were isolated, the lenses were excluded, and an immunohistochemical method was used to detect the expression of HSP27 in the LEC of each group. Results Statistical analysis was performed each week comparing LEC and HSP27 expression and the results were quite even for each group but statistics varied from week to week （P〈0.05）. Expression in the group B was clearly higher than that for the group A and C, but by the 2nd week expression in the group C was noticeably higher than that in the group A. Comparisons were made at different time intervals for each group and showed an increase in F values： during the first week F=80.99, during the second week F=261.3, during the third week F=242.6, and during the fourth week F= 719.5. There was no statistical difference for the group A （F=0.086, P=0.715）, but the differences were significant for the group B （F=563.013, P〈0.05） and group C （F=370.291, P〈0.05）. Conclusion Thermotolerance treatment can promote the expression of HSP27 in the lens epithelium and the expression is even more evident with time. HSP27 may have a protective effect on t

关 键 词：热休克蛋白类 QUERCETIN 上皮细胞 晶状体 热处理 

分 类 号：R7[医药卫生—临床医学]