苦参碱对体外培养的人角膜成纤维细胞增殖的影响  

Effect of matrine on the proliferation of human corneal fibroblasts in virto

作　　者：周珍华[1] 袁满红[1] 邓珍[1] 

机构地区：[1]南华大学附属第二医院眼科,湖南衡阳421001

出　　处：《中华眼视光学与视觉科学杂志》2010年第2期113-117,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：湖南省卫生厅课题资助项目（2006B120）

摘　　要：目的 研究苦参碱对体外培养的人角膜成纤维细胞增殖的影响.方法 将分离培养的人角膜成纤维细胞纯化后,用四唑盐比色试验（MTT比色法）分别测得培养0～8 d的人角膜成纤维细胞的光吸收值（A）,绘制出其生长曲线并计算出群体倍增时间.设立阴性对照组、溶媒对照组及阳性药物对照组（地塞米松160 mg/L）,不同浓度（10、20、40、80、160、320 mg/L）的苦参碱干预体外培养的人角膜成纤维细胞,在用药后的不同时间（24、48、72 h）,用MTT比色法测定各处理组的细胞光吸收值,计算出其抑制率,并分别对相应数据采用单因素方差分析或LSD-t检验进行统计分析.结果 体外培养的人角膜成纤维细胞的生长曲线近似＂S＂形,其群体倍增时间为52.17 h.不同浓度（10、20、40、80、160、320 mg/L）的苦参碱作用于人角膜成纤维细胞后24 h,其抑制率分别为5.08%、10.19%、18.66%、31.89%、48.93%、75.49%;作用48 h后,其抑制率分别为7.66%、15.58%、27.95%、43.45%、64.70%、89.70%;作用72 h后,其抑制率分别为11.57%、22.66%、35.50%、52.75%、70.66%、94.62%：随着作用时间的延长和浓度的增加,其抑制率均逐步增加,其抑制作用呈时间-剂量依赖性,与溶酶对照组两两比较差异均有统计学意义（P〈0.05）.地塞米松作用于人角膜成纤维细胞24、48、72 h后,抑制率分别为37.15%、50.81%、56.99%,显示出时间-效应依赖关系;且各时间段相同浓度（160 mg/L）的地塞米松与苦参碱对人角膜成纤维细胞的抑制率两两比较,苦参碱组均高于地塞米松组（P〈0.05）.结论 在10～320 mg/L浓度范围内,苦参碱对体外培养的人角膜成纤维细胞的增殖具有明显的抑制作用,其抑制作用呈时间-剂量依赖性,且160 mg/L苦参碱对人角膜成纤维细胞增殖的抑制作用强于同浓度的地塞米松.Objective To investigate the effect of matrine （the alkaloid component in sophora roots） on the inhibition of cell proliferation in human comeal fibroblasts （HCF） in vitro. Methods Purified HCF were isolated and cultured and absorbance was measured from day 0 to day 8 by MTT. The cell growth curve was plotted and the population doubling time was estimated. HCF were treated with different concentrations of matrine （10, 20, 40, 80, 160 and 320 mg/L）, cell proliferation was evaluated by MTT assay at 24 h, 48 h and 72 h. A blank control group was set up, the DXM group, using menstruum as a solvent （160 mg/L）. Results The cell growth curve of HCF approximated an ＂S＂, and the population doubling time was 52.17 h. After using different concentrations of matrine （10, 20, 40, 80, 160, 320 mg/L）, the respective inhibition ratios were 5.08%, 10.19%, 18.66%, 31.89%, 48.93% and 75.49% after 24 h, the inhibition ratios were 7.66%, 15.58%, 27.95%, 43.45%, 64.70%, 89.70%, after 48 h, and the inhibition ratios were 11.57%, 22.66%, 35.50%, 52.75%, 70.66%, 94.62% after 72 h. The inhibition ratios for the DXM group after 24 h, 48 h and 72 h were 37.15%, 50.81% and 56.99%, respectively. Conclusion For the concentrations investigated, matrine can significantly inhibit proliferation of cultured HCF in vitro. Matrine significantly suppressed the proliferation of HCF in a dose- and time-dependant positive trend, and 160 mg/L of matrine had an obviously stronger inhibitory action than DXM with the same concentrations.

关 键 词：苦参碱 角膜 成纤维细胞 人 细胞增殖 抑制 

分 类 号：R28[医药卫生—中药学]