东莨菪亭和橙皮素联用的镇痛效果定量分析  

Combination of scopoletin and hesperetin from Ipomoea Stolonifera using uniform design experimentation

作　　者：陈一村[1] 蔡聪艺[1] 冀宾[1] 高分飞[1] 张艳美[1] 黄展勤[1] 白雪婷[1] 石刚刚[1] 

机构地区：[1]汕头大学医学院药理学教研室,广东汕头515041

出　　处：《癌变．畸变．突变》2010年第3期230-232,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30973922);广东省科技计划资助项目(2008B030301240);广东省中医药局资助项目(2008162);广东省自然科学基金团队项目(9351503102000000)

摘　　要：目的:应用均匀设计法,定量分析海滩牵牛中东莨菪亭和橙皮素联用对冰醋酸所致小鼠扭体实验的镇痛效果。方法:设置5个水平的东莨菪亭(S)和橙皮素(H)联用比例和剂量,采用冰醋酸致小鼠扭体模型,观察不同组合的镇痛效果,综合数理分析结果,确定最优配方。结果:联合使用橙皮素194.5mg/kg和东莨菪亭15.36mg/kg对冰醋酸所致小鼠扭体实验可达到最佳的抑制作用。最佳剂量范围,H:175～215mg/kg;S:14～17mg/kg。结论:本实验采用均匀设计法确定了海滩牵牛中东莨菪亭和橙皮素联用对冰醋酸所致小鼠扭体实验的镇痛效果的最佳配比。均匀设计法可用于多药物联用的定量分析,但有一定的局限,其结果需经实验确证。OBJECTIVE：To analyze quantitatively the analgesic effect of scopoletin and hesperetin from Ipomoea Stolonifera in combination using the method of uniform design.METHODS：According to the uniform design method,5 different doses at 5 different proportions were administered（intra-gastric）to observe the analgesic effect to mice by writhing test.The optimal doses and proportions in combination were obtained from experimental data,by mathematical analysis and professional knowledge.RESULTS：The combination of hesperetin at 194.5 mg/kg and scopoletin at 15.36 mg/kg was appropriate.CONCLUSION：The uniform design method could be effectively applied to analyze the multidrug effects under some limited conditions,but the results must be verified by further experiment.

关 键 词：海滩牵牛 东莨菪亭 橙皮素 均匀设计法 

分 类 号：R917[医药卫生—药物分析学]