电镜下几种凋亡细胞的形态特征  

Morphological features of different apoptotic cells under the electron microscope

作　　者：沈强[1] 俞彰[1] 法京[1] 刘懿[1] 高鸿建[1] 钟慈声[1] 

机构地区：[1]复旦大学上海医学院电子显微镜中心实验室,上海200032

出　　处：《复旦学报（医学版）》2010年第3期322-325,共4页Fudan University Journal of Medical Sciences

基　　金：上海市科研计划项目(0752nm020)

摘　　要：目的观察不同种类、不同原因引起的凋亡细胞的基本形态及差异。方法用透射电镜观察原位组织和培养细胞在生理条件下和外界因素作用下产生的凋亡细胞,其中有卵巢颗粒膜细胞、烷化剂作用的光感受细胞、培养的人膀胱癌T24细胞株和肝癌细胞株。结果凋亡细胞的基本形态变化有:胞体变小、胞质浓缩、核染色质凝聚,而核膜、质膜及细胞器保存完好。各种凋亡细胞间的形态差异主要表现为核染色质凝聚的形态不同:有的凝聚染色质边移于核膜下,或呈新月形;有的核染色质凝聚成块状,散布于核内;有的呈致密凝聚,占据整个核区。结论电镜下观察到凋亡细胞的核染色质凝聚除边移外还有其他形态,正常组织中的暗细胞和坏死组织中的凝固性坏死细胞应与凋亡细胞相区别。Objective To observe common and individual morphological features of various apoptotic cells. Methods Apoptotic cell samples were prepared from organism tissues including rat ovarian follicle and retina,and from cell cultures including human bladder cancer cell line T24 and liver cancer cell line.The ultrastructure was observed under the electron microscope.The apoptotic photoreceptor cells in the rat retina were induced by alkylate,and those in the ovarian follicle happened physiologically. Results All the observed apoptotic cells showed cell body shrinkage,cytoplasm density increase and chromatin condensation,but their nuclear membrane,plasm membrane and organelles were intact.However,different apoptotic cells showed somewhat different chromatin condensation appearances.Some of them showed chromatin margination,some did a few chromatin blocks scattering in the nucleus,and others displayed a highly compact chromatin block occupying the whole nucleus. Conclusions It was observed that the chromatin condensation in apoptotic cells showed other appearances than the margination.It suggested that dark cells in a normal tissue and the pyknosis in a necrotic tissue should be carefully discriminated from apoptotic cells.

关 键 词：凋亡细胞 核染色质凝聚 核染色质边移 电子显微镜 

分 类 号：Q248[生物学—细胞生物学] Q255