绿色荧光蛋白在白念珠菌研究中的应用  

Green fluorescent protein in Candida albicans-related studies

作　　者：鹿辉[1] 曹颖瑛[2] 王彦[1] 高平挥[1] 曹永兵[1] 姜远英[1] 

机构地区：[1]第二军医大学药学院药理学教研室,上海200433 [2]第二军医大学药学院生化药学教研章,上海200433

出　　处：《第二军医大学学报》2010年第5期541-544,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30630071,30870105)~~

摘　　要：绿色荧光蛋白(green fluorescent protein,GFP)是一种来源于水母的生物发光蛋白。GFP基因作为报告基因已被广泛应用于各种生物的研究中。白念珠菌感染在临床真菌感染中占有很大的比例,因此对白念珠菌进行深入研究具有重要意义。GFP在白念珠菌的研究中已得到广泛的应用。GFP基因可与目的基因融合形成融合基因,融合基因可表达融合蛋白,该融合蛋白可发出荧光信号,进行基因表达和蛋白定位等方面的研究。本文综述了GFP在白念珠菌研究中的应用。The green fluorescent protein （GFP） is a bioluminescent protein derived from the Jellyfish Aequorea.As a reporter gene,GFP has been used in a variety of biological researches.A large proportion of the clinical fungal infections are caused by Candida albicans,making it very important for further study.GFP has also been widely used in Candida albicans-related studies.The fusion genes harboring GFP gene can yield expression of the fusion protein,which can emit green fluorescence and can be used for gene expression and protein localization.This paper reviews the application of GFP in the Candida albicans-related studies.

关 键 词：绿色荧光蛋白 白念珠菌 基因表达 蛋白定位 

分 类 号：R379.4[医药卫生—病原生物学]