蛋白精氨酸甲基转移酶在E3大鼠哮喘模型中表达变化的研究  

Expression of protein arginine N-methyltransferases in E3 rat models of acute asthma

作　　者：孙青竹[1] 焦芳芳[1] 杨旭东[1] 钟波[1] 蒋梅花[1] 李国良[1] 吕彬[1] 韩燕[1] 宁启兰[1] 张富军[1] 孙健[1] 吕社民[1] 

机构地区：[1]西安交通大学医学院遗传与分子生物学系,陕西西安710061

出　　处：《南方医科大学学报》2010年第4期716-719,共4页Journal of Southern Medical University

基　　金：国家大学生创新性实验项目(1600011263);国家自然科学基金(30630058;30801027)

摘　　要：目的观察1～6型蛋白精氨酸甲基转移酶(PRMTs)在E3大鼠急性哮喘模型肺脏和脾脏中的表达变化。方法构建卵清蛋白诱导的E3大鼠急性哮喘模型,分为对照组和哮喘组,每组各10只大鼠,病理及生化检测评估模型构建情况,半定量PCR检测1～6型PRMTs的表达差异。结果和对照组相比,哮喘组大鼠肺脏组织中PRMT1(P<0.01)、PRMT2(P<0.01)、PRMT3(P<0.05)、PRMT5(P<0.05)的表达有显著性升高,而PRMT4(P<0.05)的表达显著性降低;哮喘组大鼠脾脏组织中PRMT2(P<0.05)、PRMT5(P<0.05)的表达也出现显著性升高。结论蛋白精氨酸甲基转移酶在E3大鼠急性哮喘模型肺脏和脾脏中有差异性表达,这可能在哮喘发病的基因转录后调节过程中发挥着重要的作用。Objective To observe the expression of protein arginine N-methyltransferase （PRMT） genes in the lung and spleen of E3 rats with acute asthma. Methods E3 rats with ovalbumin-induced pulmonary inflammation were divided into two groups （n=10）, and the validity of the acute asthma model was evaluated by histological observation with HE and PAS staining and by measurement of NO production. Semi-quantitative RT-PCR was employed to detect the expressions of PRMT1-PRMT6 genes in the lung and spleen tissues of the rats. Results In the lung tissue of the asthmatic rats, the gene expressions of PRMT1 （P0.01）, PRMT2 （P0.01）, PRMT3 （P0.05） and PRMT5 （P0.05） were significantly increased, but the expression of PRMT4 gene （P0.05） was significantly decreased as compared with those in the control tissue. In the spleen tissue of the asthmatic rats, the expressions of PRMT2 （P0.05） and PRMT5 genes （P0.05） showed a significant increase as compared with those in the control rat tissue. Conclusion The gene expressions of PRMTs vary significantly between asthmatic rats and control rats, suggesting that PRMTs play an important role in the post-translational modification process of asthma-related genes.

关 键 词：蛋白精氨酸甲基转移酶 哮喘 E3大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]