肾康丸对早期糖尿病肾病大鼠肾脏AngⅡ及其受体AT_1R表达的影响  

Effects of Shenkangwan on renal expressions of angiotensin Ⅱ and its type Ⅰ receptor in rats with early diabetic nephropathy

作　　者：龙海波[1] 牛红心[1] 李小云[1] 许兆忠[1] 张辉[1] 钟娟[1] 魏连波[1] 

机构地区：[1]南方医科大学珠江医院中西医结合肾病中心,广东广州510282

出　　处：《南方医科大学学报》2010年第4期805-809,共5页Journal of Southern Medical University

基　　金：南方医科大学珠江医院院长基金(200403)

摘　　要：目的观察肾康丸对糖尿病肾病(DN)大鼠血管紧张素Ⅱ(AngⅡ)及其Ⅰ型受体(AT1R)表达的影响,探讨其对DN的肾保护作用机制。方法应用链脲佐菌素建立大鼠DN模型,将DN模型大鼠随机分为4组:DN模型对照组、肾康丸组、厄贝沙坦组、肾康丸和厄贝沙坦合用组;另设正常对照组。各组分别干预8周后,观察24h尿蛋白定量、血浆和肾组织AngⅡ含量变化,免疫组化法检测肾组织AngⅡ、AT1R的表达,RT-PCR法检测肾组织AT1RmRNA的表达,光镜观察肾脏病理改变。结果应用肾康丸干预后,DN大鼠24h尿蛋白定量、血浆及肾组织AngⅡ含量明显减少,肾组织AT1R免疫组化相对表达量及其mRNA表达水平明显降低,肾脏病理改变显著减轻。结论肾康丸可以降低DN大鼠血浆、肾组织AngⅡ水平以及肾组织AT1R含量,抑制肾组织AT1RmRNA表达,从而发挥其对DN的肾保护作用。Objective To investigate the effects of Shenkangwan on the expressions of angiotensin II （AngII） and its type I receptor （AT1R） and the renalprotection mechanism of Shenkangwan in rats with early diabetic nephropathy （DN）.Methods The rat models of DN established by a single injection of streptozotocin were randomly divided into 4 groups,namely the model group,Shenkangwan treatment group,irbesartan treatment group,and Shenkangwan and irbesartan treatment group,with normal rats as the control.All the rats received daily gavage for 8 weeks.The urinary protein quality in 24 h and plasma and renal contents of AngII were measured.The expressions of AT1R at the protein and mRNA levels in the kidney tissues were measured by immunohistochemistry and reverse transcription-polymerase chain reaction,respectively.The pathological changes of the kidney were observed microscopically.Results In DN rats,Shenkangwan reduced the urinary protein quantity in 24 h and the contents of AngII in the plasma and kidney tissues,decreased the renal expressions of AT1R protein and mRNA,and alleviated the morphological damage of the kidney.Conclusion Shenkangwan offers renalprotection against DN probably by reducing the contents of AngII in the plasma and kidney tissues and inhibiting renal AT1R expressions.

关 键 词：糖尿病肾病 血管紧张素 受体 肾康丸 厄贝沙坦 

分 类 号：R285.5[医药卫生—中药学]