近平滑念珠菌的形态转换观察  

Phenotype switching of Candida parapsilosis

作　　者：王淼淼[1] 吕雪莲[1] 刘泽虎[1] 吕桂霞[1] 沈永年[1] 刘维达[1] 

机构地区：[1]中国医学科学院北京协和医学院皮肤病研究所真菌科,南京210042

出　　处：《中国真菌学杂志》2010年第2期87-91,共5页Chinese Journal of Mycology

摘　　要：目的观察近平滑念珠菌在不同培养基的形态转换现象,以及温度对其形态转换的影响。方法收集近平滑念珠菌正常人皮肤携带株及临床致病株和标准株,接种于改良Lee培养基和含桃红B的YPD培养基,观察其不同形态转换,以及温度变化对光滑(W)与皱褶(O)形态转换的影响。结果近平滑念珠菌在Lee培养基和含桃红B的YPD培养基上,均可以出现多种形态以及一定频率W-O转换现象。在观察W向O形态转换过程中发现,与25℃培养温度相比,37℃条件下光滑菌落形态占更多的比例。结论近平滑念珠菌体外培养时存在形态转换及W-O转换现象,且于37℃时更易保持光滑形态。含桃红B的YPD培养基也可以用于基本的W-O形态转换观察。Objective To investigate the phenotype switching of Candida parapsilosis on different medium and the effect of temperature.Methods Nine isolates of C.parapsilosis collected from the skin of healthy people and clinic patients were vauinated on modified Lee＇s medium and YPD medium supplemented with phloxine B.Phenotype switching and the effect of different temperature were studied.Results High-frequency phenotypic switching and white opaque switch of C.parapsilosis were observed on both Lee＇s medium and YPD medium supplemented with phloxine B.The proportion of opaque colony under 37℃ was lower than that under 25℃ on Lee＇s medium.Conclusions C.parapsilosis is capable of high-frequency phenotypic switching and white-opaque transition with stable smooth form at 37℃.

关 键 词：念珠菌 近平滑 形态转换 光滑 皱褶 

分 类 号：R379.4[医药卫生—病原生物学]