白念珠菌YPD1基因克隆及其敲除质粒的构建  

Construction of vector and knockout plasmid of YPD1 gene of Candida albicans

作　　者：陈裕充[1,2] 温海[1] 高平挥[3] 陈江汉[1] 顾菊林[1] 郑志忠[2] 

机构地区：[1]第二军医大学附属长征医院皮肤科,上海200003 [2]复旦大学附属华山医院皮肤科,上海200040 [3]第二军医大学药学院药理学教研室,上海200433

出　　处：《中国真菌学杂志》2010年第2期97-100,共4页Chinese Journal of Mycology

摘　　要：目的构建可用于白念珠菌YPD1基因敲除的质粒。方法克隆白念珠菌YPD1基因,构建YPD1载体质粒;通过酶切反应,将p5921中标记基因URA3插入载体质粒的YPD1中,形成同源重组质粒。结果成功获得YPD1载体质粒pBluescript-YPD1和敲除质粒pBluescript-YPD1-URA3。结论所获得的质粒pBluescript-YPD1-URA3可用于白念珠菌YPD1基因的同源重组敲除。Objective To construct the plasmid for YPD1 knockout in Candida albicans.Methods The gene YPD1 of Candida albicnas was cloned in pBluescript II/SK＋.The marker gene URA3 from plasmid p5921 was inserted into the YPD1 by restriction enzyme.Results The vector plasmid pBluescript-YPD1 and knockout plasmid pBluescript-YPD1-URA3 were successfully constructed.Conclusions The plasmid pBluescript-YPD1-URA3 is helpful for homologous recombination knockout of YPD1 in Candida albicans.

关 键 词：白念珠菌 基因 质粒 

分 类 号：R379.4[医药卫生—病原生物学]