羟基红花黄色素对离体心脏功能的影响  

Effects of hydroxy safflor yellow A on cardiac function of isolated hearts

作　　者：聂培鹤[1] 张文慧[1] 金珠[1] 张琳[1] 沈国舜[1] 张镜年[1] 支建明[1] 

机构地区：[1]上海交通大学基础医学院生理学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2010年第5期540-543,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究羟基红花黄色素A(HSYA)对离体大鼠左心室收缩功能的影响。方法 42只大鼠根据实验需要随机分为正常对照组、HSYA组(1～100μmol/L)、HSYA(30μmol/L)对照组和HSYA(30μmol/L)+K+通道阻断剂组(分别为KATP阻断剂Gli、BKCa阻断剂Tea、KV阻断剂4-AP和KAch阻断剂Art),每组6只。经Langendorff离体心脏恒压灌流,依次梯度给予含有HSYA的灌流液。通过左心室内的乳胶水囊经换能器连于PowerLab生物信号采集分析系统,观察给药前后以及不同浓度HSYA对左心室收缩功能的影响,并观察不同的K+通道阻断剂对HSYA作用的影响。结果 HSYA能浓度依赖性抑制左心室收缩压、舒张末压、收缩最大速率和心率(P<0.05或P<0.01);对舒张最大速率影响不大(P>0.05)。Gli及Tea能剂量依赖性阻断HSYA的效应(P<0.05或P<0.01),4-AP及Art对HSYA的效应没有影响(P>0.05)。结论 HSYA可呈剂量依赖性抑制心脏的收缩功能和心率,其作用机制与开放KATP和BKCa通道有关。Objective To investigate the effects of hydroxy safflor yellow A（HSYA）on contractile function of left ventricle of isolated rat heart.Methods Forty-two rats were randomly divided into control group,HSYA（1-100 μmol/L）group,HSYA（30 μmol/L）control group,HSYA（30 μmol/L）＋glibenclamide（Gli）group,HSYA（30 μmol/L）＋tetraethylammonium（Tea）group,HSYA（30 μmol/L）＋4-aminopyridine（4-AP）group and HSYA（30 μmol/L）＋atropine（Art）group,with 6 rats in each group.Langendorff perfused heart preparation was made,and the isolated hearts were perfused with HSYA in a stepwise fashion.The effects on contractile function of left ventricle before and after treatment with different concentrations of HSYA were observed by PowerLab system from a fluid filled latex balloon in the left ventricle.The influence of different types of K＋ channel blockers on the effects of HSYA was also observed.ResultsHSYA reduced left ventricular systolic pressure,left ventricular end-diastolic pressure,maximum rate of increase of left ventricular pressure and heart rate in a dose-dependent manner（P0.05 or P0.01）,while had no significant effect on maximum rate of decrease of left ventricular pressure（P0.05）.Gli and Tea blocked the effects of HSYA in a concentration-dependent manner（P0.05 or P0.01）,but 4-AP and Art did not block the effects of HSYA（P0.05）.Conclusion HSYA could significantly decrease the myocardial contractility and heart rate in a dose-dependent manner,and its action mechanism might be related to the opening of ATP sensitivity K＋ channel and Ca2＋ dependent K＋ channel.

关 键 词：羟基红花黄色素A 离体心脏 心功能 K+通道 

分 类 号：S567.219[农业科学—中草药栽培]