建立荷人结肠癌裸鼠移植瘤模型及MRI成像检查  

Establishment of human colonic carcinoma (LoVo) xenograft in nude mice and magnetic resonance imaging examination

作　　者：冯仕庭[1] 孙灿辉[1] 蔡华崧[1] 周健[1] 谭国胜[1] 邱鹏新[2] 张健[3] 李子平[1] 孟悛非[1] 

机构地区：[1]中山大学附属第一医院放射科,广东省广州市510080 [2]中山大学药理学教研室,广东省广州市510080 [3]中山大学实验动物中心,广东省广州市510080

出　　处：《中国组织工程研究与临床康复》2010年第20期3696-3700,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省科技计划项目(2007B031516007)资助。课题名称:《结直肠癌免疫脂质体靶向对比剂64层螺旋CT成像研究》~~

摘　　要：背景:肿瘤移植瘤动物模型是探索和模拟肿瘤在人体内生物学行为的一种较为理想的方法,但应用MRI对模型进行评价的研究较少。目的:应用人结肠癌LoVo细胞接种裸鼠,鼠间移植传代,建立人结肠癌裸鼠移植瘤模型,并应用MRI进行初步评价。方法:采用细胞移植和瘤块移植两种方法建立裸鼠移植瘤模型,观察移植瘤大体形态和组织病理学改变,测定宿主血液中的癌胚抗原值,应用免疫组化法观察肿瘤细胞中癌胚抗原的的分布,对6只裸鼠移植瘤模型进行MRI成像检查,并测量肿瘤、肝脏、肌肉组织的T1WI和T2WI的信噪比和三者的T1,T2值。结果与结论:移植瘤的形态和功能特性与原发肿瘤基本相似,移植瘤的移植成功率为100%;6只裸鼠模型的MRI测量结果显示,其肿瘤、肝脏、肌肉的T1WI平均信噪比分别为26.19,22.71,26.62;T2WI平均信噪比分别为9.42,7.66,8.59;平均T1值分别为1039.22,907.63,1611.51ms;平均T2值分别为109.95,37.31,64.35ms。结果证实荷人结肠癌裸鼠模型的MRI成像图像清楚,组织分辨率高,测定组织的T1值和T2值可作为定量指标进行分析。BACKGROUND： The colonic carcinoma xenograft model is an ideal method for exploring and stimulating in vivo biological behavior of human, however, studies concerning evaluating models using magnetic resonance imaging （MRI） have less been reported yet. OBJECTIVE： To establish human colonic carcinoma xenograft model in nude mice with human colonic carcinoma LoVo cells inoculation and transplantation, and to evaluate the models by using MRI. METHODS： The nude mice models with colonic carcinoma were established by cell transplantation and tumor transplantation. The morphological and histopathological features of transplanted tumor were examined. Blood samples taken from the host were assayed for carcino embryonic antigen levels. Immunohistochemical localization of carcino embryonic antigen in the tumor tissues was also determined. The models （n = 6） were performed MRI examination. Signal to noise ratio （SNR） on T1 weighted imaging （T1WI） and T2 weighted imaging （T2WI）, T1 and T2 relaxation time of the tumor, liver and muscle were measured. RESULTS AND CONCLUSION： It showed that the morphological and functional characteristics of the transplanted tumors were strikingly identical to the original human tumor. The successful transplantation rate was 100%. The mean of SNR on T1WI of tumor, liver and muscle were 26.19, 22.71, and 26.62, respectively, and, the mean of SNR on T2WI were 9.42, 7.66 and 8.59, respectively. T1 relaxation time of tumor, liver and muscle were 1 039.22 ms, 907.63 ms and 1 611.51 ms, respectively, while the mean of T2 relaxation time were 109.95 ms, 37.31 ms and 64.35 ms, respectively. MRI was performed successfully in all the models with good imaging quality and high resolution. T1 and T2 relaxation time could be the quantitative index for further evaluation.

关 键 词：肿瘤移植 裸鼠 结肠肿瘤 MRI 动物模型 

分 类 号：R318[医药卫生—生物医学工程]