非诺贝特对载脂蛋白E基因敲除小鼠动脉组织CXCL16表达的影响  

Effects of Fenofibrate on CXCL16 Expression in ApoE Knockout Mice

作　　者：孙颖[1] 李敏[1] 王翠英[1] 秦明照[2] 常志文[2] 

机构地区：[1]首都医科大学附属北京友谊医院综合科,北京市100050 [2]首都医科大学附属北京同仁医院干部医疗科,北京市100730

出　　处：《中国动脉硬化杂志》2010年第3期203-207,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的趋化因子CXCL16可能在动脉粥样硬化形成中发挥重要作用,本文探讨贝特类调脂药物非诺贝特对动脉CXCL16表达的影响。方法研究对象采用载脂蛋白E基因敲除小鼠,随机分为非诺贝特组及对照组,非诺贝特干预8周后,比较两组间血脂、血清CXCL16水平;取小鼠主动脉采用医学图像分析系统测量动脉斑块大小,免疫组织化学半定量分析主动脉弓CXCL16及CXCR6的蛋白表达,实时PCR测定CXCL16mRNA。结果非诺贝特组动脉硬化程度较对照组减轻,斑块面积减小;非诺贝特组主动脉弓CXCL16和CXCR6的平均光密度值减低;非诺贝特组CXCL16mRNA表达较对照组减低(0.222±0.189比1.00±0.996,P<0.05)。结论非诺贝特可以改善载脂蛋白E基因敲除小鼠动脉粥样硬化形成,降低CXCL16及其受体CXCR6的蛋白表达,有效减低CX-CL16mRNA的表达,并且该作用独立于其调脂作用。Aim CXCL16 can be a potential player in atherogenesis.To investigate the effects of Fenofibrate on atherosclerosis and CXCL16 expression.Methods ApoE knockout mice were divided into two groups depending on treating with fenofibrate or distilled water.After 8 weeks all mice were euthanized,and ELISA was used to detect serum CXCL16 level.Aorta paraffin sections were performed with HE stain to observe and analyze atherosclerotic lesion.Expressions of CXCL16 and CXCR6 were detected by immunohistochemistry and analyzed by digital quantitative analysis software.Real-Time PCR was used to quantify CXCL16 mRNA expression.Results The atherosclerotic lesion and the expression of CXCL16 and CXCR6 were alleviated in fenofibrate group than that in control.CXCL16 mRNA expression was significantly decreased in Fenofibrate group.Conclusion Fenofibrate attenuated atherosclerosis and down-regulated CXCL16 protein and mRNA expression in ApoE knockout mice,which were independent of its cholesterol-lowering effect.

关 键 词：趋化因子 CXCL16 非诺贝特 载脂蛋白E 基因敲除 小鼠 

分 类 号：R5[医药卫生—内科学]