低氧诱导因子-1α在低氧促成肌细胞增殖中的作用  

Hypoxia enhances the proliferation of skeletal myoblasts and possible mechanism

作　　者：王凡[1] 朱玲玲[2] 陈晓萍[2] 刘国树[1] 范明[2] 

机构地区：[1]解放军总医院南楼心血管二科,北京100853 [2]军事医学科学院基础医学研究所,北京100850

出　　处：《中国应用生理学杂志》2010年第2期133-136,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金重大项目(30393130)

摘　　要：目的:探讨低氧对大鼠骨骼肌成肌细胞(SkMs)增殖的影响及低氧诱导因子(HIF-1α)在低氧促成肌细胞增殖中的相关机制。方法:采用流式细胞仪观察了3、10%O2对SkMs细胞数量和增殖指数的影响;用RT-PCR方法检测了HIF-1αmRNA的表达,用Western blot方法检测了SkMs胞浆、胞核及总HIF-1α蛋白的水平。结果:低氧组较常氧组细胞数量和增殖指数增加(P<0.05);HIF-1αmRNA、总蛋白水平在常氧组和低氧组中没有明显差异,常氧下胞浆中HIF-1α蛋白水平高于胞核内,低氧下HIF-1α蛋白水平在胞核内高于胞浆。结论:低氧能够促进SkMs增殖,HIF-1α可能是通过氧浓度调控的核转位的方式参与了低氧促SkMs的增殖。Objective：To explore the role of hypoxia in skeletal myoblasts proliferation and related mechanism.Methods：The numbers and proliferous indexes of skeletal myoblasts were detected by flow cytometer under 20%,3%,and 10% oxygen concentration.Hypoxia inducing factor 1α （HIF-1α） mRNA expression was determined by reverse transcriptase-polymerase chain reaction（RT-PCR）.HIF-1α proteinum in endochylema and intranucelus were respectively detected by Western blot.Results：The numbers and proliferous indexes were higher （P0.05） in hypoxia group than those of control group.The expression HIF-1α mRNA had no difference in hypoxia and in normal groups.The level of HIF-1α proteinum in endochylema under normoxia was more than that in intranucelus and it was opposite under hypoxia.Conclusion：Hypoxia can promote the proliferation of skeletal myoblasts.The possible mechanism of hypoxia promoting the proliferation of skeletal myoblasts might be that low oxygen concentration regulates HIF-1α nuclear translocation.

关 键 词：低氧 成肌细胞 增殖 低氧诱导因子-1Α 

分 类 号：Q253[生物学—细胞生物学]