高等植物液泡加工酶VPE家族的生物信息学分析  

Bioinformatic analysis of VPE family in higher plants

作　　者：修林春[1] 张炜[1] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095

出　　处：《南京农业大学学报》2010年第3期19-25,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30771300);国家863计划项目(2007AA10Z114)

摘　　要：利用已经分离的植物液泡加工酶(vacuolar processing enzyme,VPE)蛋白家族的Peptidase_C13结构域序列为检索序列,对拟南芥和水稻中的VPE家族成员进行序列分析,同时利用所有成员的氨基酸序列构建系统进化树,并对其中的保守序列以及信号肽区段和亚细胞定位进行了分析。结果表明:1)结合同源序列的搜索和结构域的鉴定,最终在拟南芥和水稻中确定了8个和13个VPE,其中2个拟南芥和11个水稻VPE成员为首次报道;2)蛋白系统进化分析表明,该基因家族的基本特征在拟南芥和水稻分离之前就已经形成;3)多序列联配以及MEME分析表明,Peptidase_C13结构域在VPE家族成员间高度保守;4)亚细胞定位分析表明,VPE成员定位于细胞中的不同特定位置,定位于液泡中的占多数。In this study,we used the sequence of the Peptidase_C13 domain identified from the vacuolar processing enzyme（VPE）genes in plants as queries to search for VPE genes in both Arabidopsis thaliana and Oryza sativa L.genomes.A phylogenetic tree was then constructed based on the corresponding VPE proteins from Arabidopsis and rice,and the conserved sequences,signal peptides,and subcellular localization of these proteins were analyzed.The results showed that：1）Eight and thirteen VPE from Arabidopsis thaliana and O.sativa,respectively,were identified based on the characteristic domains and the result of alignment analysis.Two and eleven of them from Arabidopsis thaliana and rice,respectively,were reported for the first time.2）The polygenetic analysis of these sequences indicated that the main character for VPE genes might have existed before the split of Arabidopsis thaliana and rice.3）The Peptidase_C13 domain was highly conserved among these proteins based on the sequence alignment analysis.4）The subcellular localization analysis indicated that different VPEs were localized in different positions in cell,but most VPEs were localized in vacuolar.

关 键 词：拟南芥 水稻 液泡加工酶 细胞程序性死亡(PCD) 生物信息学 

分 类 号：Q349[生物学—遗传学] Q943