丝瓜种质资源亲缘关系的ISSR分析  

Analysis on the phylogenetic relationship of luffa(Luffa cylindrical(L.)Roem.)germplasm based on ISSR

作　　者：苏小俊[1] 徐海[1] 陈龙正[1] 宋波[1] 袁希汉[1] 陈劲枫[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/农业部南京蔬菜遗传改良重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2010年第3期42-46,共5页Journal of Nanjing Agricultural University

基　　金：国家863计划项目(2008AA10Z150);江苏省高技术研究项目(BG2007301)

摘　　要：利用ISSR标记,对来源于不同地区的丝瓜种质资源的亲缘关系进行了分析。从80个ISSR引物中筛选出多态性强、重复性好的9个引物,对43份丝瓜种质基因组DNA进行扩增,共扩增出60条谱带,平均每个引物扩增出6.67条带,其中多态性带47个,多态性位点百分率为78.3%。丝瓜种质间遗传相似系数变化范围在0.37~0.98之间,暗示了丝瓜栽培种内的遗传基础相对狭窄。利用UPGMA聚类分析,43份丝瓜种质被划分为6个类群,类群的划分与形态学性状较吻合,而且与地理来源也有较高的相关性。Phylogenesis of 43 common luffa germplasm from different regions was analyzed with ISSR.9 primers were selected from 80 ISSR primers.60 DNA fragments were amplified from 43 samples and each primer resulted in 6.67 DNA fragments.47 fragments were polymorphic（percentage of polymorphic bands was 78.3%）.The value of Nei′s genetic similarity（GS）indexes of 43 common luffa germplasm varied from 0.37 to 0.98,suggesting that the genetic diversity among them is short.Cluster analysis with UPGMA method showed that the 43 germplasm could be divided into six groups.The results according to the pedigree relations had connections with morphological traits and regions of germplasm origin.

关 键 词：丝瓜 种质资源 亲缘关系 ISSR 

分 类 号：S642.4[农业科学—蔬菜学]