废水处理系统中活性污泥总DNA的提取  

Total DNA extraction from activated sludge in a wastewater biotreatment system

作　　者：沈国[1] 李茵[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《环境工程学报》2010年第6期1336-1340,共5页Chinese Journal of Environmental Engineering

基　　金：上海市重点学科建设项目(B604)

摘　　要：对废水脱氮除磷厌氧-缺氧-好氧系统中的活性污泥样品经过或未经预处理,比较了超声波法、玻璃珠震荡法和冻融法3种细胞裂解方法对DNA提取效果的影响。吸光度和琼脂糖凝胶电泳检测结果表明:活性污泥经TENP和PBS预处理后采用冻融法加2%SDS裂解细胞的DNA提取操作,可以得到数量多、纯度高的活性污泥DNA样品,可不经纯化直接进行PCR扩增反应。Effects of total DNA extraction from activated sludge with/without pretreatment were compared by three kinds of cell lysis methods,i.e.sonication irradiation,glass bead shaking and freeze-thawing process in an anaerobic-anoxic-aerobic denitrification-dephosphorization system.By detection of ultraviolet adsorption spectrum analysis and agarose gel electrophoresis,results showed that the DNA samples with high yield and purity were obtained by TENP and PBS pretreated,and 2%SDS and freeze-thawing lyzed,and PCR analysis was done directly without purification.

关 键 词：DNA提取 细胞裂解 活性污泥 

分 类 号：X703[环境科学与工程—环境工程]