功能化多壁碳纳米管对NHFB细胞的毒性研究  

Study of the toxicity of functionalized multi-walled carbon nanotubes to NHFB cells

作　　者：李秀娥[1] 高素莲[2] 张秋[3] 

机构地区：[1]山东大学药学院,山东济南250012 [2]山东大学环境研究院,山东济南250100 [3]山东大学化学与化工学院,山东济南250100

出　　处：《山东大学学报（理学版）》2010年第5期6-11,共6页Journal of Shandong University(Natural Science)

基　　金：国家重大基础研究发展计划(973)资助项目(2010CB933504)

摘　　要：以人的正常上皮成纤维细胞(normal human fibroblasts,NHFB)为模型,应用WST-1方法对本课题组前期合成的功能化多壁碳纳米管(functionalized multi-walled carbonnanotubes,f-MWNTs)库进行细胞毒性筛选;选取细胞毒性差异较大的几种f-WNTs,研究细胞对其摄入情况及其对细胞增殖和活性氧(reactive oxygen species,ROS)产生的影响规律。结果表明:经过不同表面修饰的多壁碳纳米管具有不同的细胞毒性;f-MWNTs能够进入细胞,且其对细胞增殖及ROS的产生均呈显著的剂量依赖关系。The cytotoxicity of the functionalized multi-walled carbon nanotubes （f-MWCNTs） library,previously synthesized by our group,to normal human fibroblasts （NHFB） was investigated by WST-1 assay.The effects of selected f-MWCNTs with different cytotoxicity on cellular uptake and proliferation,and oxidative stress in NHFB were studied.It was found that different surface modification of MWCNTs could lead to different cytotoxicity,and f-MWCNTs could be up-taken by NHFB and induce cytotoxity and reactive oxygen species in NHFB in a dose dependent manner.

关 键 词：功能化多壁碳纳米管 细胞毒性 氧化应激 

分 类 号：Q256[生物学—细胞生物学]