某部地下工程作业环境中空气颗粒物对肺脏的毒理学研究  

Research on Pulmonary Toxicity of Air Particulate Matter in Operating Environment of Underground Construction of A Unit

作　　者：胡勇[1] 邓致荣[1] 刘亮[1] 

机构地区：[1]中国人民解放军第二炮兵防护防疫环境监测队,北京市100071

出　　处：《职业与健康》2010年第12期1326-1329,共4页Occupation and Health

摘　　要：目的研究地下工程作业环境空气中颗粒物对肺脏的毒理效应。方法将采集的PM10和PM2.5制成颗粒物悬液,采用气管滴注法对6组Wistar大鼠进行染毒,每天1次,连续染毒7d。最后一次染毒结束24h后,处死大鼠,取肺泡灌洗液进行分析,检测总蛋白(TP)、巨噬细胞炎性蛋白-2(MIP-2)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)的含量或活力。肺组织作病理切片。结果随染毒剂量的增大,各项指标均出现含量或活力增高,PM10高剂量组与对照组相比,差异均有统计学意义(P<0.01),PM10中剂量组与对照组相比,差异均有统计学意义的指标有TP(P<0.05)、LDH(P<0.01)和AKP(P<0.05)。PM2.5中剂量组与对照组相比,差异有统计学意义的指标有LDH(P<0.05)和AKP(P<0.01)。而PM10低剂量组和PM2.5低剂量组与对照组相比,差异无统计学意义。结论地下工程作业环境中空气PM10和PM2.5能够引起肺部血管通透性改变和肺组织损伤。[Objective]To study toxicological effect of the air particulate matter in operating environment of underground construction.[Methods]The collected PM 10 and PM 2.5 were prepared for particulate suspension. Six groups of Wistar rats were exposed to the particulate suspension 1 time per day for continuous 7 days by tracheal instillation. After the last exposure 24 hours, the rats were killed and examined bronchoalveolar lavage fluid（BALF）. The levels and activities of total protein （TP）, MIP-2, IL-6,TNF-α, LDH, ACP and AKP were analyzed. Lung tissue was made into pathological section for observation. [Results]With the increasing of exposure dose, the levels and activities of all indicators were increased. Comparing PM 10 high-dose group with control group, the difference was significant（P0.01）. Comparing PM 10 medium-dose group with control group, the differences of TP （P0.05）, LDH（P0.01） and AKP（P0.05） were significant. Comparing PM 2.5 medium-dose group with control group, the differences of LDH（P0.05）and AKP（P0.01）were significant. While comparing PM 10 low-dose group with PM 2.5 low-dose group, the difference was not significant.[Conclusion]The PM 10 and PM 2.5 in operating environment of underground construction can cause the changing of vascular permeability and damages of lung cells.

关 键 词：地下工程 颗粒物 肺脏 毒理 

分 类 号：R114[医药卫生—卫生毒理学]