哈氏弧菌文蛤分离株WG1702培养条件优化研究  

The Optimal Culture Conditions of Bacterium Vibrio harveyi WG1702 from Clam Meretrix meretrix

作　　者：刘连生[1,2] 闫茂仓[1] 赵海泉[2] 陈倩影[2] 马爱敏[1,3] 林志华[1] 

机构地区：[1]浙江省海洋水产养殖研究所,浙江温州325005 [2]安徽农业大学生命科学院,安徽合肥230036 [3]山东农业大学动物科技学院,山东泰安271018

出　　处：《水产科学》2010年第2期79-82,共4页Fisheries Science

基　　金：国家科技支撑计划项目(2007BAD43B09);国家高技术研究发展计划项目(2006AA10A410);浙江省重大科技攻关计划项目(2006C12013)

摘　　要：于2007年9月在江苏吕泗文蛤发病高峰期,从发病文蛤体内和养殖水体中分离到1株哈氏弧菌WG1702,培养条件优化试验结果表明,菌株WG1702在32 h时达到生长高峰,最适温度为28℃,最佳初始pH为7,最佳盐度为40,最佳装液量为60 ml/150 ml,促进生长的金属离子是NH4+、Fe2+、Fe3+,最佳C、N源为葡萄糖和蛋白胨;pH、温度、不同菌龄正交试验证明最佳组合为pH 7.11,温度28℃,菌龄24 h,通过方差分析结果可以看出三因素中pH对生长影响显著(P<0.05)。A bacterial strain WG1702（Vibrio harveyi） was isolated from diseased clam Meretrix meretrix during epidemic breakdown in Lvsi harbor,Jiangsu province,in September of 2007.The optimization of culture conditions showed that the strain WG1702 reached the peak growth in 32 h culture at temperature of 28 ℃,initial pH of 7,salinity of 40,and aeration ratio of 60 ml/150 ml.The metal ions such as NH＋4,Fe2＋,and Fe3＋ were used as a cell growth enhancer,glucose as a carbon source and peptone as a nitrogen source.The orthogonal experiment for different age bacteria proved that the best growth was observed under the combination of the pH 7.11,temperature 28 ℃,and 24 h age bacteria.The variance analysis revealed that pH value had significantly influence on the growth of the bacterial strain.

关 键 词：文蛤 哈氏弧菌 最佳生长条件 

分 类 号：S917.1[农业科学—水产科学]