三疣梭子蟹促雄腺显微和亚显微结构的研究  

Microstructure and Ultrastructure of Androgenic Gland in Swimming Crab Portunus trituberculatus

作　　者：苏青[1] 朱冬发[1] 杨济芬[1] 亓一舟[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《水产科学》2010年第4期193-197,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(No40576068);浙江省科技计划项目(No2008C22048);宁波市自然科学基金资助项目(2007A610067)

摘　　要：采用解剖镜观察及组织切片和电镜技术,研究了三疣梭子蟹促雄腺的位置、形态、显微及亚显微结构。结果发现,三疣梭子蟹促雄腺1对,附着在左右射精管的表面,呈不规则索状。随着个体繁殖周期的变化,促雄腺结构各异,可划分为增殖期、合成期和分泌期3个发育时期。腺体进行全浆分泌,有A、B2种细胞类型,A型细胞具常染色质,粗面内质网和线粒体丰富,B型细胞具异染色质,细胞核固缩。处于增殖期的促雄腺量少,体积小,细胞界限清晰;合成期促雄腺体积大,呈索状,内质网和线粒体发达,A型细胞居多;分泌期分为分泌前期和分泌后期,分泌前期促雄腺体积较大,以B型细胞居多,分泌后期促雄腺体积小,部分有脱落现象,细胞器解体,细胞内存在大量空泡,有的细胞已经解体。The location,morphology,microstructure and ultrastructure of the androgenic gland in swimming crab Portunus trituberculatus were studied under zoom stereo microscope,histological section and electron microscope.The results showed that a pair of androgenic glands was found on the surface of the ejaculatory duct.The cytological development of the irregular cord-shape gland can be distinctly divided into three stages：proliferation phase,synthesis phase and secretion phase.The gland secreted in a holocrine fashion,and had two types of cells：type A,whose nuclei were rich in euchromatin,mitochondria and rough endoplasmic reticulum,and type B,which had nuclei rich in heterochromation,and showed karyopyknosis.In proliferation phase the androgenic glands were short and the glandular cells were of small amount,and had a distinct cell boundary.In synthesis phase,the androgenic glands were bigger in size than that in the proliferation phase.The glands with more type A cells appeared like a cord-shape,and had well-developed endoplasmic reticulum and mitochondria.In the prophase of the secretion phase,the androgenic glands were still big with more type B cells,and in the anaphase of the secretion phase,the androgenic glands were smaller in size,and some were broken down.There were many vacuoles in the glandular cells.

关 键 词：三疣梭子蟹 促雄腺 显微结构 亚显微结构 

分 类 号：Q243[生物学—细胞生物学]