二氢石蒜碱对大鼠心肌缺血/再灌注损伤的保护作用  

Protective Effects of Dihydrolycorine on Myocardial Ischemia-reperfusion Injury in Rats

作　　者：蒋诗琴 余光清[2] 王明华[3] 龚培力[4] 

机构地区：[1]深圳市福田区妇幼保健院药剂科,广东深圳518026 [2]深圳市宝安区疾病预防控制中心,广东深圳518101 [3]郧阳医学院附属太和医院普外Ⅲ科 [4]华中科技大学同济医学院药理系,湖北武汉430030

出　　处：《郧阳医学院学报》2010年第2期115-118,共4页Journal of Yunyang Medical College

摘　　要：目的:观察二氢石蒜碱(DL)对大鼠心肌缺血/再灌注损伤的保护作用。方法:采用大鼠在体心肌缺血/再灌注损伤模型,缺血30min,再灌注2h。于结扎左冠状动脉前降支前15min颈静脉注射DL(10,20,40mg·kg-1)治疗,假手术组和模型组则注射生理盐水对照。观察不同剂量DL对大鼠缺血/再灌注损伤后心电图ST段变化、血清肌酸激酶(CK)、乳酸脱氢酶(LDH)活性,以及心肌组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的影响。结果:与模型组相比,DL组大鼠心肌缺血/再灌注损伤后的心电图ST段抬高幅度明显减少,血清CK、LDH的活性明显减低,心肌组织MDA含量显著降低,SOD活性显著升高,以DL20及40mg/kg组作用更显著(P<0.05或P<0.01)。结论:DL对大鼠心肌缺血/再灌注损伤具有保护作用,可能与DL减少心肌组织脂质过氧化、提高心肌细胞抗氧化损伤的能力等有关。Objective To investigate the protective effects of dihydrolycorine（DL） on myocardial ischemia-reperfusion injury in rats. Methods Myocardial ischemia-reperfusion injury model was established by occluding coronary artery ischemia for 30 minutes then reperfusion for 2 hours in rat.Different dosage of Dihydrolycorine （10,20,40 mg·kg-1） were respectively given at 15 minutes before the coronary artery occlusion,and normal saline were given in both sham operation group and model group.The change of ST segment in electrocardiogram,the activities of serum lactate dehdrogenase （LDH） and creatine kinase （CK）,the enzyme activities of superoxide dismutase （SOD）,the contents of malondialdehyde （MDA） were determined and analyzed during different groups.Results Compared with model group,the ST segment elevation,the activities of CK and LDH,the contents of MDA were all significantly decreased,but the activity of SOD was significantly increased in DL groups.The effects of DL（20,40 mg·kg-1） groups were more obviously（P0.05 or P0.01）.Conclusion DL may have a protective effect on myocardial ischemia-reperfusion injury in rats,which may relate with the decreasing of myocardial lipid peroxidation and increasing the ability of myocardial against oxidant-induced injury.

关 键 词：二氢石蒜碱 心肌缺血/再灌注 心肌缺血 

分 类 号：R541[医药卫生—心血管疾病]