丙型肝炎肝组织和血清HCV RNA含量及其关系的研究  

Study on the relationship of HCV RNA between serum and liver tissue of hepatitis C

作　　者：张复春[1] 蔡晓莉[1] 黄己实 冼超[1] 吴婉芬[1] 

机构地区：[1]广州市第八人民医院传染病研究所

出　　处：《中华传染病杂志》1999年第1期15-17,共3页Chinese Journal of Infectious Diseases

基　　金：广东省重点科技攻关项目;广州市医药卫生青年基金

摘　　要：目的了解丙型肝炎肝组织和血清中丙型肝炎病毒（ＨＣＶ）ＲＮＡ的含量及其相互关系。方法采用荧光定量聚合酶链反应（ＰＣＲ）检测２４例慢性丙型肝炎患者肝组织和血清ＨＣＶＲＮＡ含量。结果肝组织中ＨＣＶＲＮＡ含量（１０８至１０１２拷贝／克）与血清病毒含量（１０５至１０９．２拷贝／毫升）呈显著正相关（Ｐ＜０．０１）。每克感染肝组织ＨＣＶＲＮＡ含量高于每毫升血清的１０２至１０４拷贝。慢性活动性肝炎肝组织和血清中ＨＣＶＲＮＡ含量显著高于慢性迁延性肝炎。动态结果显示，血清ＨＣＶＲＮＡ含量与ＡＬＴ水平呈显著正相关。结论提示慢性丙型肝炎病变程度与肝组织中ＨＣＶＲＮＡ含量有关。Objective In order to study the amounts of the hepatitis C viral RNA in the serum associated with that in the liver tissue.Methods The concentrations of HCV RNA were detected in 24 patients with chronic hepatitis C by quantitative polymerase chain reaction which was based on a Amplisensor assay. Results The amounts of HCV RNA (10 5 to 10 9.2 copies/ml) in the serum correlated with that in the liver tissue (10 8 to 10 12 copies/g) ( P <0.01). One gram of the infected liver tissue contained 10 2 to 10 4 times as many copies of the viral RNA as that of 1ml serum. Liver tissue of chronic aggressive hepatitis contained significantly higher amounts of the viral RNA than those of chronic persistent hepatitis. There was a positive correlation between the titer of HCV RNA in the serum and ALT level. Conclusion These results suggested that a significant correlation between the quantity of HCV RNA in the liver and histological acti vity of chronic hepatitis C, and the serum HCV RNA levels could be considered as a marker for monitoring intrahepatic viral RNA.

关 键 词：丙型肝炎 肝组织 血清 HCV RNA PCR 活检 

分 类 号：R512.630.4[医药卫生—内科学]