腰椎间盘突出症血瘀证与TGF-β1-509C/T基因多态性及腰椎负荷强度的关联研究  

Association of transforming growth factor β1-509C/T site gene polymorphism and lumbar burden intensity with blood stasis syndrome of lumbar intervertebral disc protrusion

作　　者：许建文[1] 钟远鸣[1] 杨光[1] 尹利军[1] 周宾宾[1] 苏洁寒[2] 张家立[1] 伍亮[1] 马显生[1] 

机构地区：[1]广西中医学院第一附属医院,南宁530023 [2]广西医科大学,南宁530022

出　　处：《中国临床新医学》2010年第5期421-424,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西自然科学基金资助项目(桂科自0991163);广西中医学院重点基金项目(ZD2006062)

摘　　要：目的探讨腰椎间盘突出症血瘀证与转化生长因子β1(TGF-β1)-509C/T基因多态性及腰椎负荷强度的关联性。方法筛选符合既定相关标准的广西汉族腰椎间盘突出症患者120例,分为血瘀证组60例与非血瘀证组60例,进行TGF-β1-509C/T基因多态性检测,并观测患者的腰椎负荷强度,分析TGF-β1-509C/T基因多态性与腰椎负荷强度的交互作用与腰椎间盘突出症中医证型的关联性。结果经非条件Lo-gistic回归Forward(LR)法分析显示广西汉族重度腰椎负荷强度者患腰椎间盘突出症血瘀证的风险是轻中度腰椎负荷强度者的3.977倍(95% CI:1.012～3.801)(P<0.05),TGF-β1基因-509C/T多态性与重度腰椎负荷强度的累积暴露发生腰椎间盘突出症血瘀证的风险是其中某单一因素的5.365倍(95% CI:1.086～2.703)(P<0.05)。结论重度腰椎负荷强度是广西汉族腰椎间盘突出症患者血瘀证的潜在危险因素之一,TGF-β1基因-509位点CT基因型与重度腰椎负荷的交互作用可增加腰椎间盘突出症血瘀型发生的可能性。Objective To investigate the relationship between the blood stasis syndrome and parts of the risk factors of lumbar intervertebral disk protrusion （LIDP）and transforming growth factor β1 （TGF-β1）-509C/T site gene polymorphisms. Methods A total of 120 Han ethnic patients from Guangxi Zhuang Autonomous Region were selected according to standard of LIDP, 60 with blood stasis syndrome and the other 60 without, then, their single nueleotide polymorphism （SNP） of TGF-β1-509C/T and lumbar burden intensity were tested, and their relationship with the blood stasis syndrome of LIDP were analyzed. Results It was showed by Logistic Regression Forward （LR） analysis that risk of syndrome blood stasis of LIDP in patients with severe lumbar burden was 3. 977 times （95% CI： 1. 012 - 3. 801 ） of that in patients with mild and middle lumbar burden （ P 〈 0.05 ）, and the risk further increased 5. 365 times （95% CI： 1. 086 - 2. 703 ） after it＇ s accumulating with TGF-β1-509C/T site gene polymorphisms in Han ethinic group in Guangxi （ P 〈 0. 05 ）. Conclusion Severe lumbar burden may be one of the risk factors of blood stasis syndrome of LIDP in Han ethnic population from Guangxi. Patients with TGF-β1 gene-509 adiponeetin CT genotype and combined severe lumbar burden are susceptible to blood stasis syndrome of LIDP.

关 键 词：腰椎间盘突出症 血瘀证 脊柱负荷 TGF-β1-509C/T基因多态性 

分 类 号：R681.5[医药卫生—骨科学]